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TREM2 antibody [HL1738]

Recombinant antibody Orthogonal Overexpression
Anti-TREM2 antibody [HL1738] used in Western Blot (WB). GTX637386
Anti-TREM2 antibody [HL1738] used in IHC (Paraffin sections) (IHC-P). GTX637386
Anti-TREM2 antibody [HL1738] used in ELISA (ELISA). GTX637386
Anti-TREM2 antibody [HL1738] used in ELISA (ELISA). GTX637386
Anti-TREM2 antibody [HL1738] used in ELISA (ELISA). GTX637386
Anti-TREM2 antibody [HL1738] used in Western Blot (WB). GTX637386
Anti-TREM2 antibody [HL1738] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637386

Cat. No. GTX637386

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1738

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA, Sandwich ELISA

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all TREM2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX637387, Detection : GTX637386 or Capture : GTX637386, Detection : GTX637387
The detection antibody requires HRP conjugation. Please contact us for the conjugation service.
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

full length human TREM2 recombinant protein.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

triggering receptor expressed on myeloid cells 2 , PLOSL2 , TREM-2 , Trem2a , Trem2b , Trem2c

Background

This gene encodes a membrane protein that forms a receptor signaling complex with the TYRO protein tyrosine kinase binding protein. The encoded protein functions in immune response and may be involved in chronic inflammation by triggering the production of constitutive inflammatory cytokines. Defects in this gene are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2012]

Database

Research Area

DATA IMAGES

Anti-TREM2 antibody [HL1738] used in Western Blot (WB). GTX637386

GTX637386 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TREM2 antibody [HL1738] (GTX637386) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TREM2 antibody [HL1738] used in IHC (Paraffin sections) (IHC-P). GTX637386

GTX637386 IHC-P Image

TREM2 antibody [HL1738] detects TREM2 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
TREM2 stained by TREM2 antibody [HL1738] (GTX637386) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TREM2 antibody [HL1738] used in ELISA (ELISA). GTX637386

GTX637386 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, full-length human TREM2 protein (1215.95-19.07 pM). Coated protein was probed with TREM2 antibody [HL1738] (GTX637386) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

Anti-TREM2 antibody [HL1738] used in ELISA (ELISA). GTX637386

GTX637386 ELISA Image

Sandwich ELISA detection of recombinant E.coli expressed, full-length human TREM2 protein using antibodies as below.

Capture: TREM2 antibody [HL1739] (GTX637387) (5 μg/mL)
Detection: HRP-conjugated TREM2 antibody [HL1738] (GTX637386) (1 μg/mL)
Please notice that GTX637386 needs to be conjugated to HRP to function as the detection antibody when paired with GTX637387. Please contact us for custom HRP-conjugated antibody.

Anti-TREM2 antibody [HL1738] used in ELISA (ELISA). GTX637386

GTX637386 ELISA Image

Sandwich ELISA detection of recombinant E.coli expressed, full-length human TREM2 protein using antibodies as below.

Capture: TREM2 antibody [HL1738] (GTX637386) (5 μg/mL)
Detection: HRP-conjugated TREM2 antibody [HL1739] (GTX637387) (1 μg/mL)
Please notice that GTX637387 needs to be conjugated to HRP to function as the detection antibody when paired with GTX637386. Please contact us for custom HRP-conjugated antibody.

Anti-TREM2 antibody [HL1738] used in Western Blot (WB). GTX637386

GTX637386 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TREM2 antibody [HL1738] (GTX637386) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-TREM2 antibody [HL1738] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637386

GTX637386 ICC/IF Image

TREM2 antibody [HL1738] detects TREM2 protein at cell membrane and cytoplasm by immunofluorescent analysis.
Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TREM2 stained by TREM2 antibody [HL1738] (GTX637386) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations