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TRF1 antibody [4E4]

Anti-TRF1 antibody [4E4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70304
Anti-TRF1 antibody [4E4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70304
Anti-TRF1 antibody [4E4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70304

Cat. No. GTX70304

Reference 11

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4E4

Isotype

 IgG1

Application

 ICC/IF

Reactivity

 Human
Package
100 μl ($399)
See all TRF1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_377594

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

telomeric repeat binding factor 1 , PIN2 , TRBF1 , TRF , TRF1 , hTRF1-AS , t-TRF1

Cellular Localization

Nucleus

Background

This gene encodes a telomere specific protein which is a component of the telomere nucleoprotein complex. This protein is present at telomeres throughout the cell cycle and functions as an inhibitor of telomerase, acting in cis to limit the elongation of individual chromosome ends. The protein structure contains a C-terminal Myb motif, a dimerization domain near its N-terminus and an acidic N-terminus. Two transcripts of this gene are alternatively spliced products. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-TRF1 antibody [4E4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70304

GTX70304 ICC/IF Image

Anti-TRF1 antibody [4E4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70304

GTX70304 ICC/IF Image

TRF1 antibody [4E4] detects TRF1 protein at telomere by immunofluorescent analysis.
Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TRF1 protein stained by TRF1 antibody [4E4] (GTX70304) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-TRF1 antibody [4E4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70304

GTX70304 ICC/IF Image

The data was published in the journal J Oncol in 2012. PMID: 22545052

REFERENCE

Application Reference

Ranjodh Sandhu et al. Proc Natl Acad Sci U S A 2021; 118 (30) The structurally conserved TELR region on shelterin protein TPP1 is essential for telomerase processivity but not recruitment.
Application : ICC/IF
Reactivity : Human
Sandhu R et al. Biochem Biophys Res Commun 2019; 512 (2) : 230-235 An N-terminal Flag-tag impairs TPP1 regulation of telomerase function.
Application : ICC/IF
Reactivity : Human

Clone Reference

Chen L et al. Nucleic Acids Res 2022; 50 (20):11619-11634 METTL3-mediated m6A modification stabilizes TERRA and maintains telomere stability.
Li X et al. Protein Cell 2021; (Epub) cGAS guards against chromosome end-to-end fusions during mitosis and facilitates replicative senescence.
Wu S et al. PLoS Genet 2020; 16 (6):e1008799 BRM-SWI/SNF chromatin remodeling complex enables functional telomeres by promoting co-expression of TRF2 and TRF1.
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REVIEW

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Application Reference
Ranjodh Sandhu et al. Proc Natl Acad Sci U S A 2021; 118 (30) The structurally conserved TELR region on shelterin protein TPP1 is essential for telomerase processivity but not recruitment.
Application : ICC/IF
Reactivity : Human
Sandhu R et al. Biochem Biophys Res Commun 2019; 512 (2):230-235 An N-terminal Flag-tag impairs TPP1 regulation of telomerase function.
Application : ICC/IF
Reactivity : Human
Cheng J et al. Cancer Sci 2015; 106 (12):1678-86 XRCC3 is a promising target to improve the radiotherapy effect of esophageal squamous cell carcinoma.
Application : ICC/IF
Reactivity : Human
Nera B et al. Nat Commun. 2015; 6:10132 Elevated levels of TRF2 induce telomeric ultrafine anaphase bridges and rapid telomere deletions.
Application : ICC/IF
Reactivity : Human
Tian XP et al. Cancer Lett 2014; the telomere/telomerase binding factor pinx1 regulates * sensitivity depending on spindle assembly checkpoint in human cervical squamous cell carcinomas.
Application : ICC/IF
Reactivity : Human
Siddiqa A et al. J Oncol 2012; 2012:806382 Modulation of telomeres in alternative lengthening of telomeres type I like human cells by the expression of werner protein and telomerase.
Application : ICC/IF
Clone Reference
Chen L et al. Nucleic Acids Res 2022; 50 (20):11619-11634 METTL3-mediated m6A modification stabilizes TERRA and maintains telomere stability.
Li X et al. Protein Cell 2021; (Epub) cGAS guards against chromosome end-to-end fusions during mitosis and facilitates replicative senescence.
Wu S et al. PLoS Genet 2020; 16 (6):e1008799 BRM-SWI/SNF chromatin remodeling complex enables functional telomeres by promoting co-expression of TRF2 and TRF1.
Zhang T et al. EMBO Rep 2017; (Epub) Looping-out mechanism for resolution of replicative stress at telomeres.
Li B et al. Mol Cell Biol 2008; 28 (6):1892-904 WRN controls formation of extrachromosomal telomeric circles and is required for TRF2DeltaB-mediated telomere shortening.
Application : ICC/IF
Reactivity : Human
SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations