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TRF1 antibody [4E4]

Anti-TRF1 antibody [4E4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70304
Anti-TRF1 antibody [4E4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70304

Cat No. GTX70304

Host

Mouse

Clonality

Monoclonal

Clone Name

4E4

Isotype

IgG1

Application

ICC/IF

Reactivity

Human
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Telomeric Repeat Binding Factor 1 , Pin2 , Trbf1 , Trf , Trf1 , Htrf1-As , T-Trf1 , Terf1

Cellular Localization

Nuclear

Background

TRF1 binds the double-stranded telomeric repeat sequence, TTAGGG. TRF1 and TRF2 are key components of vertebrate telomeres and bind to double-stranded telomeric DNA as homodimers. Dimerization involves the TRF homology (TRFH) domain, which also mediates interactions with other telomeric proteins. Despite a modest sequence identity, the 2 TRFH domains have the same entirely alpha-helical architecture, resembling a twisted horseshoe. The dimerization interfaces feature unique interactions that prevent heterodimerization. Mutational analysis of TRF1 corroborated the structural data and underscored the importance of the TRFH domain in dimerization, DNA binding, and telomere localization.

Database

Research Area

DATA IMAGES

Anti-TRF1 antibody [4E4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70304

GTX70304 ICC/IF Image

Anti-TRF1 antibody [4E4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70304

GTX70304 ICC/IF Image

TRF1 antibody [4E4] detects TRF1 protein at telomere by immunofluorescent analysis.
Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TRF1 protein stained by TRF1 antibody [4E4] (GTX70304) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

REVIEW

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SDS
PBS.pdf