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TRF1 antibody [C3], C-term

Anti-TRF1 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106349
Anti-TRF1 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106349
Anti-TRF1 antibody [C3], C-term used in Western Blot (WB). GTX106349

Cat. No. GTX106349

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all TRF1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human TRF1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241384

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

telomeric repeat binding factor 1 , PIN2 , TRBF1 , TRF , TRF1 , hTRF1-AS , t-TRF1

Cellular Localization

Nucleus , Spindle , Telomere

Background

This gene encodes a telomere specific protein which is a component of the telomere nucleoprotein complex. This protein is present at telomeres throughout the cell cycle and functions as an inhibitor of telomerase, acting in cis to limit the elongation of individual chromosome ends. The protein structure contains a C-terminal Myb motif, a dimerization domain near its N-terminus and an acidic N-terminus. Two transcripts of this gene are alternatively spliced products. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TRF1 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106349

GTX106349 ICC/IF Image

TRF1 antibody [C3], C-term detects TRF1 protein at spindle by immunofluorescent analysis.
Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TRF1 stained by TRF1 antibody [C3], C-term (GTX106349) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-TRF1 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106349

GTX106349 ICC/IF Image

TRF1 antibody [C3], C-term detects TRF1 protein at spindle by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TRF1 stained by TRF1 antibody [C3], C-term (GTX106349) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-TRF1 antibody [C3], C-term used in Western Blot (WB). GTX106349

GTX106349 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TRF1 antibody [C3], C-term (GTX106349) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Ge Y et al. Nucleic Acids Res 2016; (Epub) Preferential extension of short telomeres induced by low extracellular pH.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169