GeneTex
United States (US)

TRF1 antibody [C3], C-term

GTX106349_WB_19011506_726.jpg
GTX106349_39701_20180725_ICC_IF_19011506_756.jpg

Cat No. GTX106349

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human TRF1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

FLJ41416 antibody, PIN2 antibody, TRBF1 antibody, TRF antibody, TRF1 antibody, hTRF1-AS antibody, t-TRF1 antibody, TERF1 antibody, telomeric repeat-binding factor 1 antibody, NIMA-interacting protein 2 antibody, telomeric protein Pin2/TRF1 antibody, TTAGGG repeat-binding factor 1 antibody, telomeric repeat binding factor (NIMA-interacting) 1 antibody

Cellular Localization

Nucleus , Spindle , Telomere

Background

This gene encodes a telomere specific protein which is a component of the telomere nucleoprotein complex. This protein is present at telomeres throughout the cell cycle and functions as an inhibitor of telomerase, acting in cis to limit the elongation of individual chromosome ends. The protein structure contains a C-terminal Myb motif, a dimerization domain near its N-terminus and an acidic N-terminus. Two transcripts of this gene are alternatively spliced products. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX106349_WB_19011506_726.jpg

GTX106349 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: H1299
C: Hela
D: Hep G2 (GTX27900)
10% SDS PAGE
GTX106349 diluted at 1:1000

GTX106349_39701_20180725_ICC_IF_19011506_756.jpg

GTX106349 ICC/IF Image

TRF1 antibody [C3], C-term detects TRF1 protein at spindle by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TRF1 stained by TRF1 antibody [C3], C-term (GTX106349) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169