GeneTex
United States (US)

TRF1 antibody [TRF-78]

GTX10579_20170605_WB_19011506_445.jpg
GTX10579_20170605_ICCIF_19011506_969.jpg

Cat No. GTX10579

Host Mouse
Clonality Monoclonal
Clone Name TRF-78
Isotype IgG1
Application WB, ICC/IF, ELISA
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 2-4 μg/ml
ICC/IF Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

HeLa nuclear extract

Specificity/Sensitivity

Monoclonal Anti-TRF1 recognizes human TRF1.
PROPERTIES

Form

Liquid

Buffer

0.01M PBS pH7.4, 15 mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

~2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

human TRF1 protein produced in baculovirus.

Purification

Purified immunoglobulin

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Telomeric Repeat Binding Factor 1,Pin2,Trbf1,Trf,Trf1,Htrf1-As,T-Trf1,Terf1

Background

TRF1 binds the double-stranded telomeric repeat sequence, TTAGGG.

Database

Research Area

DATA IMAGES
GTX10579_20170605_WB_19011506_445.jpg

GTX10579 WB Image

WB analysis of (1) HeLa Nuclear extract (2) HeLa (3) HEK-293T (4) U2OS (5) HepG2 (6) P19 (7) PC12 cell lysate using GTX10579 TRF1 antibody [TRF-78] at 4 μg/mL.

GTX10579_20170605_ICCIF_19011506_969.jpg

GTX10579 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX10579 TRF1 antibody [TRF-78] at 10 μg/mL(red)with DAPI(blue). Cells were fixed and permeabilized with 4% PFA followed by 0.5% Triton X-100.

REFERENCE
Package List Price ($)
$ 289