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TRF2 antibody [4A794.15]

Anti-TRF2 antibody [4A794.15] used in Flow cytometry (FACS). GTX13579
Anti-TRF2 antibody [4A794.15] used in IHC (Paraffin sections) (IHC-P). GTX13579
Anti-TRF2 antibody [4A794.15] used in Western Blot (WB). GTX13579
Anti-TRF2 antibody [4A794.15] used in Western Blot (WB). GTX13579
Anti-TRF2 antibody [4A794.15] used in Western Blot (WB). GTX13579
Anti-TRF2 antibody [4A794.15] used in Flow cytometry (FACS). GTX13579

Cat. No. GTX13579

Host

Mouse

Clonality

Monoclonal

Clone Name

4A794.15

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA, ChIP assay, MS, PLA

Reactivity

Human, Mouse, Rat
Package
100 μg ($449)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 2 - 4 μg/ml
ICC/IF 1:10 - 1:500
IHC-P Assay dependent
IHC-Fr 5 μg/ml
FACS 0.1 μg / 10⁶ cells
IP 2 μg/ 10⁶ cells
ELISA 2 μg/ml
ChIP assay 1:10 - 1:500
MS Assay dependent
PLA Assay dependent
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Predict Reactivity

Deer(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Baculovirus expressed whole length TRF2 protein was used for immunizing mice and splenocytes used to generate the hybridoma clone (NP_005643).

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

telomeric repeat binding factor 2 , TRBF2 , TRF2

Cellular Localization

Nucleus

Background

This gene encodes a telomere specific protein, TERF2, which is a component of the telomere nucleoprotein complex. This protein is present at telomeres in metaphase of the cell cycle, is a second negative regulator of telomere length and plays a key role in the protective activity of telomeres. While having similar telomere binding activity and domain organization, TERF2 differs from TERF1 in that its N terminus is basic rather than acidic. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TRF2 antibody [4A794.15] used in Flow cytometry (FACS). GTX13579

GTX13579 FACS Image

FACS (Intracellular staining) analysis of Jurkat cells using GTX13579 TRF2 antibody [4A794.15].
Red : Primary antibody
Blue : isotype control
Shaded histogram : Cells only control

Anti-TRF2 antibody [4A794.15] used in IHC (Paraffin sections) (IHC-P). GTX13579

GTX13579 IHC-P Image

IHC-P analysis of human urinary bladder carcinoma tissue using GTX13579 TRF2 antibody [4A794.15].
Dilution : 4 μg/ml
Antigen retrieval : 10 mM sodium citrate buffer, pH 6.0

Anti-TRF2 antibody [4A794.15] used in Western Blot (WB). GTX13579

GTX13579 WB Image

WB analysis of Jurkat cell lysate using GTX13579 TRF2 antibody [4A794.15].
Dilution : 2 μg/ml

Anti-TRF2 antibody [4A794.15] used in Western Blot (WB). GTX13579

GTX13579 WB Image

WB analysis of 3T3 cell lysate using GTX13579 TRF2 antibody [4A794.15].
Dilution : 2 μg/ml

Anti-TRF2 antibody [4A794.15] used in Western Blot (WB). GTX13579

GTX13579 WB Image

WB analysis of HeLa cell lysate using GTX13579 TRF2 antibody [4A794.15].

Anti-TRF2 antibody [4A794.15] used in Flow cytometry (FACS). GTX13579

GTX13579 FACS Image

FACS analysis of Jurkat cells using GTX13579 TRF2 antibody [4A794.15].
Red : Primary antibody
Blue : isotype control
Antibody amount : 0.1μg

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 449