APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 2 - 4 μg/ml |
| 1:10 - 1:500 |
| Assay dependent |
| 5 μg/ml |
| 0.1 μg / 10⁶ cells |
| 2 μg/ 10⁶ cells |
| 2 μg/ml |
| 1:10 - 1:500 |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Deer(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Baculovirus expressed whole length TRF2 protein was used for immunizing mice and splenocytes used to generate the hybridoma clone (NP_005643).
Purification
Protein G purified
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
telomeric repeat binding factor 2 , TRBF2 , TRF2
Cellular Localization
Nucleus
Background
This gene encodes a telomere specific protein, TERF2, which is a component of the telomere nucleoprotein complex. This protein is present at telomeres in metaphase of the cell cycle, is a second negative regulator of telomere length and plays a key role in the protective activity of telomeres. While having similar telomere binding activity and domain organization, TERF2 differs from TERF1 in that its N terminus is basic rather than acidic. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-TRF2 antibody [4A794.15] used in Flow cytometry (FACS). GTX13579](/templates/default/js/lightbox/images/loading.gif "Anti-TRF2 antibody [4A794.15] used in Flow cytometry (FACS). GTX13579")
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GTX13579 FACS Image
FACS (Intracellular staining) analysis of Jurkat cells using GTX13579 TRF2 antibody [4A794.15].
Red : Primary antibody
Blue : isotype control
Shaded histogram : Cells only control
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GTX13579 IHC-P Image
IHC-P analysis of human urinary bladder carcinoma tissue using GTX13579 TRF2 antibody [4A794.15].
Dilution : 4 μg/ml
Antigen retrieval : 10 mM sodium citrate buffer, pH 6.0
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GTX13579 WB Image
WB analysis of Jurkat cell lysate using GTX13579 TRF2 antibody [4A794.15].
Dilution : 2 μg/ml
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GTX13579 WB Image
WB analysis of 3T3 cell lysate using GTX13579 TRF2 antibody [4A794.15].
Dilution : 2 μg/ml
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GTX13579 WB Image
WB analysis of HeLa cell lysate using GTX13579 TRF2 antibody [4A794.15].
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GTX13579 FACS Image
FACS analysis of Jurkat cells using GTX13579 TRF2 antibody [4A794.15].
Red : Primary antibody
Blue : isotype control
Antibody amount : 0.1μg
|
REFERENCE
There are currently no references for TRF2 antibody [4A794.15] (GTX13579). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for TRF2 antibody [4A794.15] (GTX13579). Be the first to share your experience with this product.
![Anti-TRF2 antibody [4A794.15] used in Flow cytometry (FACS). GTX13579](/upload/website/prouct_img/small/GTX13579/GTX13579_68_FACS_18121410_256.jpg "Anti-TRF2 antibody [4A794.15] used in Flow cytometry (FACS). GTX13579")
![Anti-TRF2 antibody [4A794.15] used in IHC (Paraffin sections) (IHC-P). GTX13579](/upload/website/prouct_img/small/GTX13579/GTX13579_401_IHC-P_18121410_859.jpg "Anti-TRF2 antibody [4A794.15] used in IHC (Paraffin sections) (IHC-P). GTX13579")
![Anti-TRF2 antibody [4A794.15] used in Western Blot (WB). GTX13579](/upload/website/prouct_img/small/GTX13579/GTX13579_944_WB_18121410_760.jpg "Anti-TRF2 antibody [4A794.15] used in Western Blot (WB). GTX13579")
![Anti-TRF2 antibody [4A794.15] used in Western Blot (WB). GTX13579](/upload/website/prouct_img/small/GTX13579/GTX13579_942_WB_18121410_891.jpg "Anti-TRF2 antibody [4A794.15] used in Western Blot (WB). GTX13579")
![Anti-TRF2 antibody [4A794.15] used in Western Blot (WB). GTX13579](/upload/website/prouct_img/small/GTX13579/GTX13579_943_WB_18121410_632.jpg "Anti-TRF2 antibody [4A794.15] used in Western Blot (WB). GTX13579")
![Anti-TRF2 antibody [4A794.15] used in Flow cytometry (FACS). GTX13579](/upload/website/prouct_img/small/GTX13579/GTX13579_12_FACS_18121410_729.jpg "Anti-TRF2 antibody [4A794.15] used in Flow cytometry (FACS). GTX13579")
![Anti-TRF2 antibody [4A794.15] used in Flow cytometry (FACS). GTX13579](/upload/website/prouct_img/normal/GTX13579/GTX13579_68_FACS_18121410_256.jpg "Anti-TRF2 antibody [4A794.15] used in Flow cytometry (FACS). GTX13579")
![Anti-TRF2 antibody [4A794.15] used in IHC (Paraffin sections) (IHC-P). GTX13579](/upload/website/prouct_img/normal/GTX13579/GTX13579_401_IHC-P_18121410_859.jpg "Anti-TRF2 antibody [4A794.15] used in IHC (Paraffin sections) (IHC-P). GTX13579")
![Anti-TRF2 antibody [4A794.15] used in Western Blot (WB). GTX13579](/upload/website/prouct_img/normal/GTX13579/GTX13579_944_WB_18121410_760.jpg "Anti-TRF2 antibody [4A794.15] used in Western Blot (WB). GTX13579")
![Anti-TRF2 antibody [4A794.15] used in Western Blot (WB). GTX13579](/upload/website/prouct_img/normal/GTX13579/GTX13579_942_WB_18121410_891.jpg "Anti-TRF2 antibody [4A794.15] used in Western Blot (WB). GTX13579")
![Anti-TRF2 antibody [4A794.15] used in Western Blot (WB). GTX13579](/upload/website/prouct_img/normal/GTX13579/GTX13579_943_WB_18121410_632.jpg "Anti-TRF2 antibody [4A794.15] used in Western Blot (WB). GTX13579")
![Anti-TRF2 antibody [4A794.15] used in Flow cytometry (FACS). GTX13579](/upload/website/prouct_img/normal/GTX13579/GTX13579_12_FACS_18121410_729.jpg "Anti-TRF2 antibody [4A794.15] used in Flow cytometry (FACS). GTX13579")