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TRF2 antibody

Anti-TRF2 antibody used in Western Blot (WB). GTX132780
Anti-TRF2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132780

Cat No. GTX132780

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

A549 , H1299 , HCT-116

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.470 mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TRF2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

telomeric repeat binding factor 2 , TRBF2 , TRF2

Cellular Localization

Nucleus

Background

This gene encodes a telomere specific protein, TERF2, which is a component of the telomere nucleoprotein complex. This protein is present at telomeres in metaphase of the cell cycle, is a second negative regulator of telomere length and plays a key role in the protective activity of telomeres. While having similar telomere binding activity and domain organization, TERF2 differs from TERF1 in that its N terminus is basic rather than acidic. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-TRF2 antibody used in Western Blot (WB). GTX132780

GTX132780 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TRF2 antibody (GTX132780) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Multiple bands were observed 66 kDa, and 68 kDa. It is possibly due to alternative splicing.
Reference:PMID: 27329590

Anti-TRF2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132780

GTX132780 ICC/IF Image

TRF2 antibody detects TRF2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TRF2 stained by TRF2 antibody (GTX132780) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar= 10 μm.

REFERENCE

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REVIEW

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