GeneTex
United States (US)

TRH Receptor antibody [C2C3], C-term

GTX100030_39549_IFA_19011506_691.jpg
GTX100030_39549_IHC_19011506_497.jpg
GTX100030_WB_19011506_655.jpg
GTX100030_39549_20171201_IHC-P_R_19011506_638.jpg

Cat No. GTX100030

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

293T , A431 , Raji

Predict Reactivity

Mouse, Sheep, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human TRH Receptor. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MGC141920 antibody, TRHR antibody, thyroliberin receptor antibody, thyrotropin-releasing hormone receptor antibody

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

Thyrotropin-releasing hormone (TRH; MIM 275120), a small neuropeptide, is widely distributed throughout the central and peripheral nervous system as well as in extraneural tissues. The peptide is synthesized in the hypothalamus and transported by the portal vascular system to the anterior pituitary where it acts on thyrotropic and lactotropic cells to promote secretion of TSH and prolactin, respectively. Thyrotropin-releasing hormone receptor is a G protein-coupled receptor that activates the inositol phospholipid-calcium-protein kinase C transduction pathway upon the binding of TRH. The TRHR gene is expressed in the thyrotrope cells of the anterior pituitary.[supplied by OMIM]

Database

DATA IMAGES
GTX100030_39549_IFA_19011506_691.jpg

GTX100030 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using TRHR(GTX100030) antibody at 1:500 dilution.

GTX100030_39549_IHC_19011506_497.jpg

GTX100030 IHC-P Image

Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using TRHR(GTX100030) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100030_WB_19011506_655.jpg

GTX100030 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
10% SDS PAGE
GTX100030 diluted at 1:2000

GTX100030_39549_20171201_IHC-P_R_19011506_638.jpg

GTX100030 IHC-P Image

TRH Receptor antibody [C2C3], C-term detects TRH Receptor protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
TRH Receptor stained by TRH Receptor antibody [C2C3], C-term (GTX100030) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SDS
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 319
$ 169