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TRIB1 antibody [N2C3]

Anti-TRIB1 antibody [N2C3] used in Western Blot (WB). GTX111960
Anti-TRIB1 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX111960

Cat. No. GTX111960

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all TRIB1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

A431 , HeLa

Predict Reactivity

Mouse, Rat, Bovine, Dog, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.41 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TRIB1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952383

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tribbles pseudokinase 1 , C8FW , GIG-2 , GIG2 , SKIP1 , TRB-1 , TRB1

Background

Interacts with MAPK kinases and regulates activation of MAP kinases. May not display kinase activity.

Database

Research Area

DATA IMAGES

Anti-TRIB1 antibody [N2C3] used in Western Blot (WB). GTX111960

GTX111960 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TRIB1 antibody [N2C3] (GTX111960) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TRIB1 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX111960

GTX111960 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using TRIB1(GTX111960) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

S Soubeyrand et al. Sci Rep 2023; 13 (1) : 9320 Regulation of TRIB1 abundance in hepatocyte models in response to proteasome inhibition
Application : WB, IP
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169