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TRIB3 antibody [N3C3]

Anti-TRIB3 antibody [N3C3] used in Western Blot (WB). GTX113991
Anti-TRIB3 antibody [N3C3] used in Western Blot (WB). GTX113991
Anti-TRIB3 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX113991
Anti-TRIB3 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113991

Cat No. GTX113991

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

HepG2 , HUVEC , HUVEC treat with Sphingosine 1-phosphate

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TRIB3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2038166

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tribbles pseudokinase 3 , C20orf97 , NIPK , SINK , SKIP3 , TRB3

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a putative protein kinase that is induced by the transcription factor NF-kappaB. The encoded protein is a negative regulator of NF-kappaB and can also sensitize cells to TNF- and TRAIL-induced apoptosis. In addition, this protein can negatively regulate the cell survival serine-threonine kinase AKT1. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TRIB3 antibody [N3C3] used in Western Blot (WB). GTX113991

GTX113991 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TRIB3 antibody [N3C3] (GTX113991) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-TRIB3 antibody [N3C3] used in Western Blot (WB). GTX113991

GTX113991 WB Image

Sample (15 μg of whole cell lysate)
A: HUVEC
GTX113991 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TRIB3 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX113991

GTX113991 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 Xenograft, using TRB-3 (GTX113991) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TRIB3 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113991

GTX113991 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TRB-3 (GTX113991) antibody at 1:200 dilution.

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169