APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1 - 2 μg/mL |
| 5 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Mouse Kidney Tissue Lysate(GTX27936)
Specificity/Sensitivity
At least two isoforms of TRB3 are known to exist; this antibody will detect both isoforms.
PROPERTIES
Form
Liquid
Buffer
PBS with 0.02% sodium azide.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
TRIB3 antibody was raised against a 17 amino acid peptide near the center of human TRIB3.The immunogen is located within amino acids 100 - 150 of TRIB3.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
tribbles pseudokinase 3 , C20orf97 , NIPK , SINK , SKIP3 , TRB3
Cellular Localization
Nucleus
Background
The protein encoded by this gene is a putative protein kinase that is induced by the transcription factor NF-kappaB. The encoded protein is a negative regulator of NF-kappaB and can also sensitize cells to TNF- and TRAIL-induced apoptosis. In addition, this protein can negatively regulate the cell survival serine-threonine kinase AKT1. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX31610 WB Image
WB analysis of mouse kidney tissue lysate using GTX31610 TRIB3 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml
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GTX31610 IHC-P Image
IHC-P analysis of mouse liver tissue using GTX31610 TRIB3 antibody.
Working concentration : 20 μg/ml
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GTX31610 IHC-P Image
IHC-P analysis of mouse liver tissue using GTX31610 TRIB3 antibody.
Working concentration : 5 μg/ml
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REFERENCE
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REVIEW
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