GeneTex
United States (US)

TRIF antibody [C3], C-term

GTX104744_39701_IFA_19011506_789.jpg
GTX104744_WB_19011506_491.jpg

Cat No. GTX104744

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

76 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.01mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human TRIF. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Toll Like Receptor Adaptor Molecule 1,Iiae6,Myd88-3,Prvtirb,Ticam-1,Trif,Ticam1

Background

TRIF, or TICAM1, is a Toll/IL1R (MIM 147810) (TIR) domain-containing adaptor molecule, like MYD88 (MIM 602170) and TIRAP (MIM 606252), that induces interferon-beta (IFNB; MIM 147640), specifically interacts with TLR3 (MIM 603029), and activates nuclear factor kappa-B (NFKB; see MIM 164011).[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX104744_39701_IFA_19011506_789.jpg

GTX104744 ICC/IF Image

TRIF antibody [C3], C-term detects TRIF protein at cytosol by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde for 10 min.
Green: TRIF protein stained by TRIF antibody [C3], C-term (GTX104744) diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX104744_WB_19011506_491.jpg

GTX104744 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
GTX104744 diluted at 1:500

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169