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TRIF antibody [C3], C-term

Anti-TRIF antibody [C3], C-term used in Western Blot (WB). GTX104744
Anti-TRIF antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104744

Cat. No. GTX104744

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all TRIF products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

76 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.01 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human TRIF. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241389

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

toll like receptor adaptor molecule 1 , IIAE6 , MyD88-3 , PRVTIRB , TICAM-1 , TRIF

Background

TRIF, or TICAM1, is a Toll/IL1R (MIM 147810) (TIR) domain-containing adaptor molecule, like MYD88 (MIM 602170) and TIRAP (MIM 606252), that induces interferon-beta (IFNB; MIM 147640), specifically interacts with TLR3 (MIM 603029), and activates nuclear factor kappa-B (NFKB; see MIM 164011).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-TRIF antibody [C3], C-term used in Western Blot (WB). GTX104744

GTX104744 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
GTX104744 diluted at 1:500

Anti-TRIF antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104744

GTX104744 ICC/IF Image

TRIF antibody [C3], C-term detects TRIF protein at cytosol by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde for 10 min.
Green: TRIF protein stained by TRIF antibody [C3], C-term (GTX104744) diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

Application Reference

Mlcochova P et al. Cell Rep 2020; 30 (12) : 3972-3980.e5 TLR4-Mediated Pathway Triggers Interferon-Independent G0 Arrest and Antiviral SAMHD1 Activity in Macrophages.
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169