APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.5-2μg/ml |
| 6-12μg/ml |
| 10 μg/ml |
| <1:16000 |
|
Note :
IHC-P
Human Brain (Cortex) shows cytoplasm stainning in neuronal cell bodies.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89871-PEP).
Product Note
This antibody is expected to recognise both reported isoforms (NP_056086.2; NP_001123539.1). Cross-reactivity with TRIM3 has been demonstrated.
Predict Reactivity
Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence DSGNHCFKVYRYLQ, from the C Terminus of the protein sequence according to NP_056086.2; NP_001123539.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
tripartite motif containing 2 , CMT2R , RNF86
Cellular Localization
Cytoplasm
Background
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic filaments. It plays a neuroprotective role and functions as an E3-ubiquitin ligase in proteasome-mediated degradation of target proteins. Mutations in this gene can cause early-onset axonal neuropathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]
Database
Research Area
DATA IMAGES
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GTX89871 WB Image
WB analysis of rat brain lysate using GTX89871 TRIM2 antibody, C-term.
Dilution : 0.5μg/ml
Loading : 35μg protein in RIPA buffer
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GTX89871 IHC-P Image
IHC-P analysis of human cortex using GTX89871 TRIM2 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 6.3μg/ml
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GTX89871 FACS Image
FACS analysis of PFA-fixed Jurkat cells using GTX89871 TRIM2 antibody, C-term.
Blue : Primary antibody
Black: Unimmunized goat IgG
Permeabilization : 0.5% Triton
Dilution : 10 μg/mL
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REFERENCE
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REVIEW
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