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TRIM23 antibody [N1], N-term

Anti-TRIM23 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX100057
Anti-TRIM23 antibody [N1], N-term used in Western Blot (WB). GTX100057
Anti-TRIM23 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100057
Anti-TRIM23 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100057

Cat. No. GTX100057

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all TRIM23 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.67 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human TRIM23. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1080934

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tripartite motif containing 23 , ARD1 , ARFD1 , RNF46

Cellular Localization

Endomembrane system , Golgi apparatus membrane , Lysosome membrane

Background

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein is also a member of the ADP ribosylation factor family of guanine nucleotide-binding family of proteins. Its carboxy terminus contains an ADP-ribosylation factor domain and a guanine nucleotide binding site, while the amino terminus contains a GTPase activating protein domain which acts on the guanine nucleotide binding site. The protein localizes to lysosomes and the Golgi apparatus. It plays a role in the formation of intracellular transport vesicles, their movement from one compartment to another, and phopholipase D activation. Three alternatively spliced transcript variants for this gene have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TRIM23 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX100057

GTX100057 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using TRIM23(GTX100057) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TRIM23 antibody [N1], N-term used in Western Blot (WB). GTX100057

GTX100057 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TRIM23 antibody [N1], N-term (GTX100057) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TRIM23 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100057

GTX100057 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using TRIM23(GTX100057) antibody (Green) at 1:500 dilution.

Anti-TRIM23 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100057

GTX100057 ICC/IF Image

TRIM23 antibody [N1], N-term detects TRIM23 protein at cytoplasm by immunofluorescent analysis.
Samples: HCT116 cells mock (left) and treated with 30 μM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TRIM23 protein stained by TRIM23 antibody [N1], N-term (GTX100057) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.

REFERENCE

Application Reference

Kahle JJ et al. Hum Mol Genet 2011; 20 (3) : 510-27 Comparison of an expanded ataxia interactome with patient medical records reveals a relationship between macular degeneration and ataxia.
Application : IHC-Fr
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 399
$ 169