GeneTex
  • Country / Location Selection

United States (US)

TRIM23 antibody [N1], N-term

Anti-TRIM23 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX100057
Anti-TRIM23 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100057
Anti-TRIM23 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100057
Anti-TRIM23 antibody [N1], N-term used in Western Blot (WB). GTX100057

Cat No. GTX100057

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Positive Control

HeLaS3

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human TRIM23. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

tripartite motif containing 23,ARD1,ARFD1,RNF46

Cellular Localization

Endomembrane system , Golgi apparatus membrane , Lysosome membrane

Background

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein is also a member of the ADP ribosylation factor family of guanine nucleotide-binding family of proteins. Its carboxy terminus contains an ADP-ribosylation factor domain and a guanine nucleotide binding site, while the amino terminus contains a GTPase activating protein domain which acts on the guanine nucleotide binding site. The protein localizes to lysosomes and the Golgi apparatus. It plays a role in the formation of intracellular transport vesicles, their movement from one compartment to another, and phopholipase D activation. Three alternatively spliced transcript variants for this gene have been described. [provided by RefSeq]

Database

DATA IMAGES
Anti-TRIM23 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX100057

GTX100057 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using TRIM23(GTX100057) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TRIM23 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100057

GTX100057 ICC/IF Image

TRIM23 antibody [N1], N-term detects TRIM23 protein at cytoplasm by immunofluorescent analysis.
Samples: HCT116 cells mock (left) and treated with 30 μM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TRIM23 protein stained by TRIM23 antibody [N1], N-term (GTX100057) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.

Anti-TRIM23 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100057

GTX100057 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using TRIM23(GTX100057) antibody (Green) at 1:500 dilution.

Anti-TRIM23 antibody [N1], N-term used in Western Blot (WB). GTX100057

GTX100057 WB Image

Sample(30 μg of whole cell lysate)
A:HeLa S3(GTX14654)
10% SDS PAGE
GTX100057 diluted at 1:1000

REFERENCE
REVIEW
There are currently no reviews for TRIM23 antibody [N1], N-term (GTX100057). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 319
$ 169