TRIM25 antibody immunoprecipitates TRIM25 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of TRIM25 protein by 2 μg of TRIM25 antibody (GTX116209) 7.5% SDS-PAGE The immunoprecipitated TRIM25 protein was detected by TRIM25 antibody (GTX116209) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Sample (30 ug of whole cell lysate)

A: A549

7.5% SDS PAGE

GTX116209 diluted at 1:1000

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TRIM25 antibody (GTX116209) diluted at 1:500.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using TRIM25(GTX116209) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

TRIM25 antibody detects TRIM25 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde for 10 min.
Green: TRIM25 protein stained by TRIM25 antibody (GTX116209) diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.