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TRIM25 antibody

Anti-TRIM25 antibody used in Western Blot (WB). GTX66657
Anti-TRIM25 antibody used in Western Blot (WB). GTX66657

Cat. No. GTX66657

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399)
See all TRIM25 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide, corresponding to amino acids 360-418 of Human EFP.

Purification

Purified by antigen-affinity chromatography
From serum

Purity

> 95% (by SDS-PAGE)

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tripartite motif containing 25 , EFP , RNF147 , Z147 , ZNF147

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-TRIM25 antibody used in Western Blot (WB). GTX66657

GTX66657 WB Image

WB analysis of various samples using GTX66657 TRIM25 antibody.
Lane1 : H1792 whole cell lysate(10ug)
Lane2 : HepG2 whole cell lysate(10ug)
Lane3 : MCF-7 whole cell lysate(10ug)
Lane4 : L02 whole cell lysate(10ug)
Dilution : 1:1000

Anti-TRIM25 antibody used in Western Blot (WB). GTX66657

GTX66657 WB Image

WB analysis of various samples using GTX66657 TRIM25 antibody.
Lane1 : HEK293T whole cell lysate(40ug)
Lane2 : MCF-7 whole cell lysate(40ug)
Dilution : 1:500

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399