GeneTex
United States (US)

TRIM27 antibody

GTX102048_42102_20150710_IP_19011506_276.jpg
GTX102048_39645_20141017_WB_19011506_309.jpg
GTX102048_42102_20160317_WB_R_testis_19011506_755.jpg
GTX102048_39645_IFA_19011506_460.jpg
GTX102048_42102_20150522_WB_19011506_571.jpg

Cat No. GTX102048

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

K562 , THP-1 , HL-60 , U87-MG , SK-N-SH , IMR32 , rat testis

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.66 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TRIM27. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tripartite Motif Containing 27,Rfp,Rnf76,Trim27

Cellular Localization

Nucleus , Cytoplasm

Background

This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the nuclear matrix. It interacts with the enhancer of polycomb protein and represses gene transcription. It is also thought to be involved in the differentiation of male germ cells. Fusion of the N-terminus of this protein with the truncated C-terminus of the RET gene product has been shown to result in production of the ret transforming protein. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX102048_42102_20150710_IP_19011506_276.jpg

GTX102048 IP Image

Immunoprecipitation of TRIM27 protein from Jurkat whole cell extracts using 5 μg of TRIM27 antibody (GTX102048).
Western blot analysis was performed using TRIM27 antibody (GTX102048).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX102048_39645_20141017_WB_19011506_309.jpg

GTX102048 WB Image

TRIM27 antibody detects TRIM27 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TRIM27 antibody (GTX102048) diluted by 1:500.

GTX102048_42102_20160317_WB_R_testis_19011506_755.jpg

GTX102048 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with TRIM27 antibody (GTX102048) diluted at 1:500.

GTX102048_39645_IFA_19011506_460.jpg

GTX102048 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using TRIM27(GTX102048) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

GTX102048_42102_20150522_WB_19011506_571.jpg

GTX102048 WB Image

TRIM27 antibody detects TRIM27 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TRIM27 antibody (GTX102048) diluted at a dilution of 1:500.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169