Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using TRIM27(GTX102048) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

TRIM27 antibody detects TRIM27 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TRIM27 antibody (GTX102048) diluted by 1:500.

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with TRIM27 antibody (GTX102048) diluted at 1:500.

Immunoprecipitation of TRIM27 protein from Jurkat whole cell extracts using 5 μg of TRIM27 antibody (GTX102048).
Western blot analysis was performed using TRIM27 antibody (GTX102048).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

TRIM27 antibody detects TRIM27 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TRIM27 antibody (GTX102048) diluted at a dilution of 1:500.