GeneTex
United States (US)

TRIM27 antibody

Anti-TRIM27 antibody used in Western Blot (WB). GTX33559
Anti-TRIM27 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33559
Anti-TRIM27 antibody used in IHC (Paraffin sections) (IHC-P). GTX33559
Anti-TRIM27 antibody used in IHC (Paraffin sections) (IHC-P). GTX33559

Cat No. GTX33559

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:10 - 1:100
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

58 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 214-513 of human TRIM27 (NP_006501.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tripartite Motif Containing 27,Rfp,Rnf76,Trim27

Background

This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the nuclear matrix. It interacts with the enhancer of polycomb protein and represses gene transcription. It is also thought to be involved in the differentiation of male germ cells. Fusion of the N-terminus of this protein with the truncated C-terminus of the RET gene product has been shown to result in production of the ret transforming protein. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-TRIM27 antibody used in Western Blot (WB). GTX33559

GTX33559 WB Image

WB analysis of various samples using GTX33559 TRIM27 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-TRIM27 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33559

GTX33559 ICC/IF Image

ICC/IF analysis of U2OS cell using GTX33559 TRIM27 antibody.
Blue : DAPI

Anti-TRIM27 antibody used in IHC (Paraffin sections) (IHC-P). GTX33559

GTX33559 IHC-P Image

IHC-P analysis of mouse stomach tissue using GTX33559 TRIM27 antibody.
Dilution : 1:100

Anti-TRIM27 antibody used in IHC (Paraffin sections) (IHC-P). GTX33559

GTX33559 IHC-P Image

IHC-P analysis of human esophagus tissue using GTX33559 TRIM27 antibody.
Dilution : 1:100

Package List Price ($)
$ 329