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TRIM27 antibody

Anti-TRIM27 antibody used in IHC (Paraffin sections) (IHC-P). GTX33559
Anti-TRIM27 antibody used in IHC (Paraffin sections) (IHC-P). GTX33559
Anti-TRIM27 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33559
Anti-TRIM27 antibody used in Western Blot (WB). GTX33559

Cat. No. GTX33559

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all TRIM27 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:10 - 1:100
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 214-513 of human TRIM27 (NP_006501.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tripartite motif containing 27 , RFP , RNF76

Cellular Localization

Nucleus,Cytoplasm

Background

This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the nuclear matrix. It interacts with the enhancer of polycomb protein and represses gene transcription. It is also thought to be involved in the differentiation of male germ cells. Fusion of the N-terminus of this protein with the truncated C-terminus of the RET gene product has been shown to result in production of the ret transforming protein. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-TRIM27 antibody used in IHC (Paraffin sections) (IHC-P). GTX33559

GTX33559 IHC-P Image

IHC-P analysis of mouse stomach tissue using GTX33559 TRIM27 antibody.
Dilution : 1:100

Anti-TRIM27 antibody used in IHC (Paraffin sections) (IHC-P). GTX33559

GTX33559 IHC-P Image

IHC-P analysis of human esophagus tissue using GTX33559 TRIM27 antibody.
Dilution : 1:100

Anti-TRIM27 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33559

GTX33559 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX33559 TRIM27 antibody.
Blue : DAPI

Anti-TRIM27 antibody used in Western Blot (WB). GTX33559

GTX33559 WB Image

WB analysis of various sample lysates using GTX33559 TRIM27 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399