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TRIM29 antibody [C1C3]

Anti-TRIM29 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX115749
Anti-TRIM29 antibody [C1C3] used in Western Blot (WB). GTX115749
Anti-TRIM29 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX115749
Anti-TRIM29 antibody [C1C3] used in Immunoprecipitation (IP). GTX115749

Cat. No. GTX115749

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, IP

Reactivity

Human, Mouse
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.99 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TRIM29. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10632868

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tripartite motif containing 29 , ATDC

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene belongs to the TRIM protein family. It has multiple zinc finger motifs and a leucine zipper motif. It has been proposed to form homo- or heterodimers which are involved in nucleic acid binding. Thus, it may act as a transcriptional regulatory factor involved in carcinogenesis and/or differentiation. It may also function in the suppression of radiosensitivity since it is associated with ataxia telangiectasia phenotype. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TRIM29 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX115749

GTX115749 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using TRIM29(GTX115749) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TRIM29 antibody [C1C3] used in Western Blot (WB). GTX115749

GTX115749 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
10% SDS PAGE
GTX115749 diluted at 1:2000

Anti-TRIM29 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX115749

GTX115749 IHC-P Image

TRIM29 antibody [C1C3] detects TRIM29 protein at cytoplasm in mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
TRIM29 antibody [C1C3] (GTX115749) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TRIM29 antibody [C1C3] used in Immunoprecipitation (IP). GTX115749

GTX115749 IP Image

TRIM29 antibody immunoprecipitates TRIM29 protein in IP experiments. IP Sample: A431 whole cell lysate/extract A : 30 μg whole cell lysate/extract of TRIM29 protein expressing A431 cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of TRIM29 by 2.5 μg of TRIM29 antibody (GTX115749) 10% SDS-PAGE The immunoprecipitated TRIM29 protein was detected by TRIM29 antibody (GTX115749) diluted at 1:2000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169