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TRIM29 antibody [C1C3]

Orthogonal
Anti-TRIM29 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX115749
Anti-TRIM29 antibody [C1C3] used in Western Blot (WB). GTX115749
Anti-TRIM29 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX115749
Anti-TRIM29 antibody [C1C3] used in Immunoprecipitation (IP). GTX115749

Cat. No. GTX115749

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all TRIM29 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.99 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TRIM29. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10632868

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tripartite motif containing 29 , ATDC

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene belongs to the TRIM protein family. It has multiple zinc finger motifs and a leucine zipper motif. It has been proposed to form homo- or heterodimers which are involved in nucleic acid binding. Thus, it may act as a transcriptional regulatory factor involved in carcinogenesis and/or differentiation. It may also function in the suppression of radiosensitivity since it is associated with ataxia telangiectasia phenotype. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TRIM29 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX115749

GTX115749 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using TRIM29(GTX115749) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TRIM29 antibody [C1C3] used in Western Blot (WB). GTX115749

GTX115749 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
10% SDS PAGE
GTX115749 diluted at 1:2000

Anti-TRIM29 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX115749

GTX115749 IHC-P Image

TRIM29 antibody [C1C3] detects TRIM29 protein at cytoplasm in mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
TRIM29 antibody [C1C3] (GTX115749) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TRIM29 antibody [C1C3] used in Immunoprecipitation (IP). GTX115749

GTX115749 IP Image

TRIM29 antibody immunoprecipitates TRIM29 protein in IP experiments. IP Sample: A431 whole cell lysate/extract A : 30 μg whole cell lysate/extract of TRIM29 protein expressing A431 cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of TRIM29 by 2.5 μg of TRIM29 antibody (GTX115749) 10% SDS-PAGE The immunoprecipitated TRIM29 protein was detected by TRIM29 antibody (GTX115749) diluted at 1:2000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

REFERENCE

Application Reference

Ali SR et al. Mol Ther Nucleic Acids 2022; 30 : 30-47 Functional high-throughput screen identifies microRNAs that promote butyrate-induced death in colorectal cancer cells.
Application : WB
Reactivity : Human
Zeng SX et al. Oncol Rep 2017; 38 (3) : 1645-1654 High expression of TRIM29-(ATDC) contributes to poor prognosis and tumor metastasis by inducing epithelial-mesenchymal transition in osteosarcoma.
Application : IHC-P
Fujimura K et al. J Biol Chem 2015; 290 (50) : 29907-19 Eukaryotic Translation Initiation Factor 5A (EIF5A) Regulates Pancreatic Cancer Metastasis by Modulating RhoA and Rho-associated Kinase (ROCK) Protein Expression Levels.
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169