GeneTex
United States (US)

TRIM29 antibody, Internal

GTX88145_1035_IHC-P_18122102_902.jpg
GTX88145_132_ICC-IF_18122102_448.jpg
GTX88145_131_ICC-IF_18122102_313.jpg
GTX88145_58_FACS_18122102_881.jpg
GTX88145_2472_WB_18122102_694.jpg

Cat No. GTX88145

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, FACS
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-1μg/ml
ICC/IF 10μg/ml
IHC-P 3.75μg/ml
FACS Assay dependent

Note :

ICC/IF
Strong expression of the protein seen in the Cytoplasm and Intermediate filament of A431 cells.Strong expression of the protein seen in the Nucleus of U2OS cells.

IHC-P
Human Skin shows staining of subnuclear structures and cytoplasm in keratinocytes.

Not tested in other applications.

Calculated MW

66 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88145-PEP).

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-EKDRIKSFTTNE, from the internal region of the protein sequence according to NP_036233.2; NP_001317311.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tripartite Motif Containing 29,Atdc,Trim29

Cellular Localization

Colocalizes with intermediate filaments.

Background

The protein encoded by this gene belongs to the TRIM protein family. It has multiple zinc finger motifs and a leucine zipper motif. It has been proposed to form homo- or heterodimers which are involved in nucleic acid binding. Thus, it may act as a transcriptional regulatory factor involved in carcinogenesis and/or differentiation. It may also function in the suppression of radiosensitivity since it is associated with ataxia telangiectasia phenotype. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES
GTX88145_1035_IHC-P_18122102_902.jpg

GTX88145 IHC-P Image

IHC-P analysis of human skin using GTX88145 TRIM29 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75µg/ml

GTX88145_132_ICC-IF_18122102_448.jpg

GTX88145 ICC/IF Image

ICC/IF analysis of PFA fixed U2OS cells using GTX88145 TRIM29 antibody, Internal.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 10µg/ml

GTX88145_131_ICC-IF_18122102_313.jpg

GTX88145 ICC/IF Image

ICC/IF analysis of PFA fixed A431 cells using GTX88145 TRIM29 antibody, Internal.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 10µg/ml

GTX88145_58_FACS_18122102_881.jpg

GTX88145 FACS Image

FACS analysis of PFA fixed A431 cells using GTX88145 TRIM29 antibody, Internal.
Blue : Primary antibody
Black : Isotype control
Permeabilization : 0.5% Triton
Dilution : 10µg/ml

GTX88145_2472_WB_18122102_694.jpg

GTX88145 WB Image

WB analysis of peripheral blood mononucleocyte (A) and HeLa (B) cell lysate using GTX88145 TRIM29 antibody, Internal.
Dilution : 1µg/ml
Loading : 35µg protein in RIPA buffer

Package List Price ($)
$ 329