GeneTex
United States (US)

TRIM29 antibody [TRIM29/1041]

GTX35147_20170712_IHC-P_18121410_761.jpg
GTX35147_20170712_IHC-P_1_18121410_369.jpg

Cat No. GTX35147

Host Mouse
Clonality Monoclonal
Clone Name TRIM29/1041
Isotype IgG2a
Application ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 0.5-1ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM Citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

66 kDa. ( Note )

Specificity/Sensitivity

It recognizes a 66kDa protein, which is identified as Tripartite motif-containing protein 29 (TRIM29). It interacts with the intermediate filament protein vimentin, a substrate for the PKC family of protein kinases, and with hPKCI-1, an inhibitor of the PKCs. TRIM29 protein contains both zinc finger and leucine zipper motifs, suggesting that the it may form homodimers and possibly associate with DNA. High expression of TRIM29 has been reported in gastric cancer and pancreatic cancer, and correlates with enhanced tumor growth and lymph node metastasis. TRIM29 is also able to distinguish lung squamous cell carcinoma from lung adenocarcinoma with ~90% positive accuracy, when used in a panel with TTF-1, p63, CK5/6, and Napsin-A antibodies.
PROPERTIES

Form

Liquid

Buffer

Prepared in 1mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fragment (126 Amino acid residues between aa 1-200) of human TRIM29 protein

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tripartite Motif Containing 29,Atdc,Trim29

Background

The protein encoded by this gene belongs to the TRIM protein family. It has multiple zinc finger motifs and a leucine zipper motif. It has been proposed to form homo- or heterodimers which are involved in nucleic acid binding. Thus, it may act as a transcriptional regulatory factor involved in carcinogenesis and/or differentiation. It may also function in the suppression of radiosensitivity since it is associated with ataxia telangiectasia phenotype. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES
GTX35147_20170712_IHC-P_18121410_761.jpg

GTX35147 IHC-P Image

Formalin-fixed, paraffin-embedded human Lung Squamous Cell Carcinoma stained with TRIM29 Monoclonal Antibody (TRIM29/1041).

GTX35147_20170712_IHC-P_1_18121410_369.jpg

GTX35147 IHC-P Image

Formalin-fixed, paraffin-embedded human Esophageal carcinoma stained with TRIM29 Monoclonal Antibody (TRIM29/1041).

Package List Price ($)
$ 329