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TRIM32 antibody

Overexpression
Anti-TRIM32 antibody used in Western Blot (WB). GTX113937
Anti-TRIM32 antibody used in Western Blot (WB). GTX113937
Anti-TRIM32 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113937
Anti-TRIM32 antibody used in IHC (Paraffin sections) (IHC-P). GTX113937

Cat. No. GTX113937

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all TRIM32 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

Positive Control

DDDDK-human TRIM32-transfected 293T cells

Predict Reactivity

Rat, Bovine, Cat, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TRIM32. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2038168

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tripartite motif containing 32 , BBS11 , HT2A , LGMD2H , LGMDR8 , TATIP

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. The protein has also been localized to the nucleus, where it interacts with the activation domain of the HIV-1 Tat protein. The Tat protein activates transcription of HIV-1 genes. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TRIM32 antibody used in Western Blot (WB). GTX113937

GTX113937 WB Image

TRIM32 antibody detects TRIM32 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
7.5% SDS-PAGE
TRIM32 antibody (GTX113937) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TRIM32 antibody used in Western Blot (WB). GTX113937

GTX113937 WB Image

Overexpression

TRIM32 antibody detects TRIM32 protein by western blot analysis.
A. 5 μg 293T whole cell lysate/extract
B. 5 μg whole cell lysate/extract of DDDDK-human TRIM32-transfected 293T cells
7.5% SDS-PAGE
TRIM32 antibody (GTX113937) dilution: 1:20000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TRIM32 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113937

GTX113937 ICC/IF Image

TRIM32 antibody detects TRIM32 protein at cytoplasm by immunofluorescent analysis.
Sample: A375 cells were fixed in ice-cold MeOH for 5 min.
Green: TRIM32 protein stained by TRIM32 antibody (GTX113937) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-TRIM32 antibody used in IHC (Paraffin sections) (IHC-P). GTX113937

GTX113937 IHC-P Image

TRIM32 antibody detects TRIM32 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
TRIM32 antibody (GTX113937) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Prajapati P et al. Cell Signal 2020; 76 : 109777 TRIM32 regulates mitochondrial mediated ROS levels and sensitizes the oxidative stress induced cell death.
Application : WB
Reactivity : Human
Wang M et al. Cell Death Differ 2019; Trim32 suppresses cerebellar development and tumorigenesis by degrading Gli1/sonic hedgehog signaling.
Application : WB
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169