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TRIP15 antibody

Anti-TRIP15 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116463
Anti-TRIP15 antibody used in IHC (Paraffin sections) (IHC-P). GTX116463
Anti-TRIP15 antibody used in Western Blot (WB). GTX116463
Anti-TRIP15 antibody used in IHC (Paraffin sections) (IHC-P). GTX116463
Anti-TRIP15 antibody used in Western Blot (WB). GTX116463

Cat. No. GTX116463

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all TRIP15 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , NIH-3T3 , JC , BCL-1

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TRIP15. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11168504

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

COP9 signalosome subunit 2 , ALIEN , CSN2 , SGN2 , TRIP15

Cellular Localization

Cytoplasm , Nucleus

Database

Research Area

DATA IMAGES

Anti-TRIP15 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116463

GTX116463 ICC/IF Image

TRIP15 antibody detects TRIP15 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TRIP15 protein stained by TRIP15 antibody (GTX116463) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-TRIP15 antibody used in IHC (Paraffin sections) (IHC-P). GTX116463

GTX116463 IHC-P Image

TRIP15 antibody detects TRIP15 protein at cytosol and nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
TRIP15 antibody (GTX116463) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TRIP15 antibody used in Western Blot (WB). GTX116463

GTX116463 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
C: HeLa
D: HepG2
10% SDS PAGE
GTX116463 diluted at 1:10000

Anti-TRIP15 antibody used in IHC (Paraffin sections) (IHC-P). GTX116463

GTX116463 IHC-P Image

TRIP15 antibody detects TRIP15 protein at cytosol and nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
TRIP15 antibody (GTX116463) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TRIP15 antibody used in Western Blot (WB). GTX116463

GTX116463 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
10% SDS PAGE
GTX116463 diluted at 1:5000

REFERENCE

Application Reference

Lin M et al. Phytother Res 2018; (Epub) Quercetin improves postpartum hypogalactia in milk-deficient mice via stimulating prolactin production in pituitary gland.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169