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TRMT12 antibody

Anti-TRMT12 antibody used in Western Blot (WB). GTX32015
Anti-TRMT12 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32015

Cat No. GTX32015

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, ELISA

Reactivity

Human, Mouse
Package
100 μg ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5 μg/mL
ICC/IF 10 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

K-562 Cell Slide(GTX25535) , K562 Cell Lysate(GTX27911)

Specificity/Sensitivity

This antibody is predicted to not cross-react with other TYW protein family members. At least two isoforms are known to exist; this antibody will detect both isoforms

PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

TRMT12 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human TRMT12.The immunogen is located within the first 50 amino acids of TRMT12.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tRNA methyltransferase 12 homolog , TRM12 , TYW2

Background

Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TRMT12 is the human homolog of a yeast gene essential for yW synthesis (Noma and Suzuki, 2006).[supplied by OMIM, Mar 2008]

Database

DATA IMAGES

Anti-TRMT12 antibody used in Western Blot (WB). GTX32015

GTX32015 WB Image

WB analysis of K562 cell lysate in (A) the absence and (B) the presence of blocking peptide using GTX32015 TRMT12 antibody.
Working concentration : 0.5 μg/ml

Anti-TRMT12 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32015

GTX32015 ICC/IF Image

ICC/IF analysis of K562 cells using GTX32015 TRMT12 antibody.
Working concentration : 10 μg/ml

REFERENCE

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Package List Price ($)
$ 329