GeneTex
United States (US)

TRPC4 antibody, Internal

GTX88652_2203_WB_18121411_127.jpg
GTX88652_891_IHC-P_18121411_367.jpg
GTX88652_890_IHC-P_18121411_221.jpg

Cat No. GTX88652

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.3-1μg/ml
IHC-P 4.75μg/ml
Not tested in other applications.

Calculated MW

112 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88652-PEP).

Specificity/Sensitivity

This antibody is expected to recognise all six reported isoforms (NP_057263.1; NP_003297.1; NP_001129427.1; NP_001129429.1; NP_001129428.1; NP_001129430.1).

Predict Reactivity

Bovine, Dog, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Concentration

0.50mg/ml

Antigen Species

Human

Immunogen

Peptide with sequence C-KHAKEEDSSIDYD, from the internal region (near C Terminus) of the protein sequence according to NP_057263.1; NP_003297.1; NP_001129427.1; NP_001129429.1; NP_001129428.1; NP_001129430.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

HTRP4 Antibody , TRP4 Antibody

Cellular Localization

Enhanced insertion into the cell membrane after activation of the EGF receptor.

Background

This gene encodes a member of the canonical subfamily of transient receptor potential cation channels. The encoded protein forms a non-selective calcium-permeable cation channel that is activated by Gq-coupled receptors and tyrosine kinases, and plays a role in multiple processes including endothelial permeability, vasodilation, neurotransmitter release and cell proliferation. Single nucleotide polymorphisms in this gene may be associated with generalized epilepsy with photosensitivity. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011]
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Database

Research Area

DATA IMAGES
GTX88652_2203_WB_18121411_127.jpg

GTX88652 WB Image

WB analysis of rat brain lysate using GTX88652 TRPC4 antibody, Internal.
Dilution : 0.3µg/ml
Loading : 35µg protein in RIPA buffer

GTX88652_891_IHC-P_18121411_367.jpg

GTX88652 IHC-P Image

IHC-P analysis of human placenta using GTX88652 TRPC4 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 4.75µg/ml

GTX88652_890_IHC-P_18121411_221.jpg

GTX88652 IHC-P Image

IHC-P analysis of human kidney using GTX88652 TRPC4 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 4.75µg/ml