APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.3-1μg/ml |
| 4.75μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX88652-PEP).
Product Note
This antibody is expected to recognise all six reported isoforms (NP_057263.1; NP_003297.1; NP_001129427.1; NP_001129429.1; NP_001129428.1; NP_001129430.1).
Predict Reactivity
Bovine, Dog, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-KHAKEEDSSIDYD, from the internal region (near C Terminus) of the protein sequence according to NP_057263.1; NP_003297.1; NP_001129427.1; NP_001129429.1; NP_001129428.1; NP_001129430.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
transient receptor potential cation channel subfamily C member 4 , HTRP-4 , HTRP4 , TRP4
Cellular Localization
Membrane; Multi-pass membrane protein , Cell membrane; Multi-pass membrane protein
Background
This gene encodes a member of the canonical subfamily of transient receptor potential cation channels. The encoded protein forms a non-selective calcium-permeable cation channel that is activated by Gq-coupled receptors and tyrosine kinases, and plays a role in multiple processes including endothelial permeability, vasodilation, neurotransmitter release and cell proliferation. Single nucleotide polymorphisms in this gene may be associated with generalized epilepsy with photosensitivity. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011]
Database
Research Area
DATA IMAGES
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GTX88652 IHC-P Image
IHC-P analysis of human kidney using GTX88652 TRPC4 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 4.75μg/ml
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GTX88652 WB Image
WB analysis of rat brain lysate using GTX88652 TRPC4 antibody, Internal.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer
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GTX88652 IHC-P Image
IHC-P analysis of human placenta using GTX88652 TRPC4 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 4.75μg/ml
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REFERENCE
There are currently no references for TRPC4 antibody, Internal (GTX88652). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for TRPC4 antibody, Internal (GTX88652). Be the first to share your experience with this product.
