GeneTex
United States (US)

TRPC6 antibody [C3], C-term

Orthogonal
GTX104730_39652_20181109_IHC-P-FL_M_19022001_679.jpg
GTX104730_WB_19011506_369.jpg
GTX104730_39652_20160114_WB_Fraction_19011506_415.jpg

Cat No. GTX104730

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

106 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Raji , A549 , A549 membrane fraction extract
PROPERTIES

Form

Liquid

Buffer

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human TRPC6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Transient Receptor Potential Cation Channel Subfamily C Member 6,Fsgs2,Trp6,Trpc6

Cellular Localization

Membrane; Multi-pass membrane protein

Background

The protein encoded by this gene forms a receptor-activated calcium channel in the cell membrane. The channel is activated by diacylglycerol and is thought to be under the control of a phosphatidylinositol second messenger system. Activation of this channel occurs independently of protein kinase C and is not triggered by low levels of intracellular calcium. Defects in this gene are a cause of focal segmental glomerulosclerosis 2 (FSGS2). [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX104730_39652_20181109_IHC-P-FL_M_19022001_679.jpg

GTX104730 IHC-P Image

TRPC6 antibody [C3], C-term detects TRPC6 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
Green: TRPC6 stained by TRPC6 antibody [C3], C-term (GTX104730) diluted at 1:500.
Red: NF-H, a neuron marker, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX104730_WB_19011506_369.jpg

GTX104730 WB Image

Sample(30 μg of whole cell lysate)
A:HeLa S3(GTX14654)
7.5% SDS PAGE
GTX104730 diluted at 1:3000

GTX104730_39652_20160114_WB_Fraction_19011506_415.jpg

GTX104730 WB Image

Orthogonal

A549 whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TRPC6 antibody (GTX104730) diluted at 1:3000.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169