GeneTex

  • Country / Location Selection

United States (US)

TRPC6 antibody [N1N3]

Orthogonal
Anti-TRPC6 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113858
Anti-TRPC6 antibody [N1N3] used in Western Blot (WB). GTX113858

Cat. No. GTX113858

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all TRPC6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

106 kDa. ( Note )

Positive Control

A549 membrane fraction

Predict Reactivity

Bovine, Guinea pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.84 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Intracellular domain of human TRPC6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10719642

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transient receptor potential cation channel subfamily C member 6 , FSGS2 , TRP6

Background

The protein encoded by this gene forms a receptor-activated calcium channel in the cell membrane. The channel is activated by diacylglycerol and is thought to be under the control of a phosphatidylinositol second messenger system. Activation of this channel occurs independently of protein kinase C and is not triggered by low levels of intracellular calcium. Defects in this gene are a cause of focal segmental glomerulosclerosis 2 (FSGS2). [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TRPC6 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113858

GTX113858 IHC-P Image

TRPC6 antibody [N1N3] detects TRPC6 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
Green: TRPC6 stained by TRPC6 antibody [N1N3] (GTX113858) diluted at 1:500.
Red: NF-H, a neuron marker, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TRPC6 antibody [N1N3] used in Western Blot (WB). GTX113858

GTX113858 WB Image

Orthogonal

A549 whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TRPC6 antibody (GTX113858) diluted at 1:1000.

REFERENCE

Application Reference

Hayes HL et al. Mol Cell Biol 2013; 33 (20) : 4017-29 Pdx-1 activates islet α- and β-cell proliferation via a mechanism regulated by transient receptor potential cation channels 3 and 6 and extracellular signal-regulated kinases 1 and 2.
Application : WB
Reactivity : Rat
Submit a Reference

REVIEW

There are currently no reviews for TRPC6 antibody [N1N3] (GTX113858). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169