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TRPM2 antibody [N1N2-2], N-term

Comparison Orthogonal
Anti-TRPM2 antibody [N1N2-2], N-term used in Western Blot (WB). GTX112252
Anti-TRPM2 antibody [N1N2-2], N-term used in IHC (Paraffin sections) (IHC-P). GTX112252
Anti-TRPM2 antibody [N1N2-2], N-term used in Western Blot (WB). GTX112252
Anti-TRPM2 antibody [N1N2-2], N-term used in IHC (Paraffin sections) (IHC-P). GTX112252

Cat. No. GTX112252

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all TRPM2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

171 kDa. ( Note )

Positive Control

HepG2 membrane extract , HepG2 , HepG2 membrane extract

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.38 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Intracellular domain of human TRPM2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952408

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transient receptor potential cation channel subfamily M member 2 , EREG1 , KNP3 , LTRPC2 , LTrpC-2 , NUDT9H , NUDT9L1 , TRPC7

Cellular Localization

Membrane; Multi-pass membrane protein

Background

The protein encoded by this gene is a calcium-permeable cation channel that is regulated by free intracellular ADP-ribose. The encoded protein is activated by oxidative stress and confers susceptibility to cell death. Several alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TRPM2 antibody [N1N2-2], N-term used in Western Blot (WB). GTX112252

GTX112252 WB Image

Comparison Orthogonal

HepG2 whole cell and membrane extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with TRPM2 antibody [N1N2-2], N-term (GTX112252) diluted at 1:500 and competitor's antibody (ACC-043) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-TRPM2 antibody [N1N2-2], N-term used in IHC (Paraffin sections) (IHC-P). GTX112252

GTX112252 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MDAMB468 xenograft, using TRPM2(GTX112252) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TRPM2 antibody [N1N2-2], N-term used in Western Blot (WB). GTX112252

GTX112252 WB Image

Orthogonal

HepG2 whole cell and membrane extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TRPM2 antibody [N1N2-2], N-term (GTX112252) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-TRPM2 antibody [N1N2-2], N-term used in IHC (Paraffin sections) (IHC-P). GTX112252

GTX112252 IHC-P Image

TRPM2 antibody [N1N2-2], N-term detects TRPM2 protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
TRPM2 antibody [N1N2-2], N-term (GTX112252) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

MacGlashan D Jr et al. PLoS One 2015; 10 (5) : e0126435 Expression profiling of human basophils: modulation by cytokines and secretagogues.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169