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TRPM7 antibody [S74-25]

Anti-TRPM7 antibody [S74-25] used in Western Blot (WB). GTX41997
Anti-TRPM7 antibody [S74-25] used in Immunohistochemistry (IHC). GTX41997
Anti-TRPM7 antibody [S74-25] used in Immunohistochemistry (IHC). GTX41997

Cat. No. GTX41997

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 S74-25

Isotype

 IgG1

Application

 WB, ICC/IF, IP, IHC

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all TRPM7 products

APPLICATION

Application Note

We recommend a working dilution of 1-10ug/mL (WB), 0.1-1.0ug/mL (Perox) (IHC/ICC), 1.0-10ug/mL (IF).

Calculated MW

212 kDa. ( Note )

Product Note

No cross-reactivity against TrpM6

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Fusion protein amino acids 1817-1863 (C- terminus) of mouse TrpM7, accession number Q923J1

Purification

Protein G Purified

Conjugation

Unconjugated

RRID

AB_10729129

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transient receptor potential cation channel, subfamily M, member 7 , 2310022G15Rik , 4833414K03Rik , 5033407O22Rik , CHAK , CHAK1 , LTrpC-7 , Ltpr7 , Ltrpc7 , TRPPLIK

Cellular Localization

Membrane

Database

Research Area

DATA IMAGES

Anti-TRPM7 antibody [S74-25] used in Western Blot (WB). GTX41997

GTX41997 WB Image

Western blot analysis of TrpM7 tested in a human cell line mix at 1:1000 dilution of GTX41997.

Anti-TRPM7 antibody [S74-25] used in Immunohistochemistry (IHC). GTX41997

GTX41997 IHC Image

IHC analysis of TrpM7 in human hippocampal tissues using GTX41997

Anti-TRPM7 antibody [S74-25] used in Immunohistochemistry (IHC). GTX41997

GTX41997 IHC Image

IHC analysis of TrpM7 tested on mouse brain using a dilution of GTX41997. Strong positivity of the nuclear membrane of every cell.

REFERENCE

Application Reference

Starostina I et al. Sci Rep 2021; 11 (1) : 17893 Distinct calcium regulation of TRPM7 mechanosensitive channels at plasma membrane microdomains visualized by FRET-based single cell imaging.
Application : ICC/IF
Reactivity : Human
Kim TJ et al. Adv Sci (Weinh) 2019; 6 (4) : 1801290 Matrix Rigidity-Dependent Regulation of Ca2+ at Plasma Membrane Microdomains by FAK Visualized by Fluorescence Resonance Energy Transfer.
Application : ICC/IF
Reactivity : Human
Liu YS et al. Sci Rep 2015; 5 : 16522 Mechanosensitive TRPM7 mediates shear stress and modulates osteogenic differentiation of mesenchymal stromal cells through Osterix pathway.
Application : ICC/IF
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 399