APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
Not tested in other applications.
Calculated MW
Positive Control
H1299
Predict Reactivity
Mouse, Rat, Bovine, Dog, Guinea pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.56 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the Intracellular domain of human TRPM8. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1952410
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
transient receptor potential cation channel subfamily M member 8 , LTRPC6 , LTrpC-6 , TRPP8 , trp-p8
Cellular Localization
Membrane; Multi-pass membrane protein
Background
Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonists menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing.
Database
Research Area
DATA IMAGES
![Anti-TRPM8 antibody [N2C1], Internal used in Western Blot (WB). GTX111818](/templates/default/js/lightbox/images/loading.gif "Anti-TRPM8 antibody [N2C1], Internal used in Western Blot (WB). GTX111818")
|
GTX111818 WB Image
Sample (30 ug of whole cell lysate)
A: H1299
7.5% SDS PAGE
GTX111818 diluted at 1:500
|
REFERENCE
There are currently no references for TRPM8 antibody [N2C1], Internal (GTX111818). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for TRPM8 antibody [N2C1], Internal (GTX111818). Be the first to share your experience with this product.
![Anti-TRPM8 antibody [N2C1], Internal used in Western Blot (WB). GTX111818](/upload/website/prouct_img/small/GTX111818/GTX111818_WB_w_23060500_547.webp "Anti-TRPM8 antibody [N2C1], Internal used in Western Blot (WB). GTX111818")
![Anti-TRPM8 antibody [N2C1], Internal used in Western Blot (WB). GTX111818](/upload/website/prouct_img/normal/GTX111818/GTX111818_WB_w_23060500_547.webp "Anti-TRPM8 antibody [N2C1], Internal used in Western Blot (WB). GTX111818")