Knockdown/Knockout validation was supported by references (PMID: 35525986)
Predict Reactivity
Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.8 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide SDVDGTTYDFAHC, corresponding to amino acid residues 917-929 (3rd extracellular loop) of human TRPM8 (Accession : Q7Z2W7).
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
RRID
AB_2887902
Note
Knockdown/Knockout validation was supported by references (PMID: 35525986).
TARGET
Synonyms
transient receptor potential cation channel subfamily M member 8 , LTRPC6 , LTrpC-6 , TRPP8 , trp-p8
IHC-Fr analysis of rat DRG tissue using GTX54866 TRPM8 antibody. TRPM8 is expressed in DRG neurons. Hoechst 33342 is used as the counterstain. Dilution : 1:100
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GTX54866 ICC/IF LCI Image
ICC/IF(Panel A) and Live cell imaging(Panel B) analysis of rat dorsal root ganglion (DRG) cells using GTX54866 TRPM8 antibody. Blue : Hoechst 33342 for nuclear staining Dilution : 1:500 for Panel A ; 1:50 for Panel B
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GTX54866 IHC-Fr Image
IHC-Fr analysis of rat DRG tissue using GTX54866 TRPM8 antibody. Cell nuclei were stained with DAPI (blue). Panel A : TRPM8 labeling (green). Panel B : The same section was then labeled for TrkA (purple). Panel C : Merge of A and B demonstrates co-localization of TRPM8 and TrkA in rat DRG (arrows). Dilution : 1:300
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GTX54866 WB Image
WB analysis of DU145 (lanes 1, 3), LNCaP (lanes 2, 4), and mouse-TRPM8 transfected HEK-293 (lanes 5, 6) cell lysates using GTX54866 TRPM8 antibody preincubated with or without immunogen peptide. Dilution : 1:200
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GTX54866 ICC/IF LCI Image
Live cell imaging(Panel A) and ICC/IF(Panel B) analysis of LNCaP cells using GTX54866 TRPM8 antibody. Dilution : 1:100
