Live cell imaging(Panel A) and ICC/IF(Panel B) analysis of LNCaP cells using GTX54866 TRPM8 antibody.
Dilution : 1:100

WB analysis of DU145 (lanes 1, 3), LNCaP (lanes 2, 4), and mouse-TRPM8 transfected HEK-293 (lanes 5, 6) cell lysates using GTX54866 TRPM8 antibody preincubated with or without immunogen peptide.
Dilution : 1:200

IHC-Fr analysis of rat DRG tissue using GTX54866 TRPM8 antibody. Cell nuclei were stained with DAPI (blue).
Panel A : TRPM8 labeling (green).
Panel B : The same section was then labeled for TrkA (purple).  
Panel C : Merge of A and B demonstrates co-localization of TRPM8 and TrkA in rat DRG (arrows).
Dilution : 1:300

ICC/IF(Panel A) and Live cell imaging(Panel B) analysis of rat dorsal root ganglion (DRG) cells using GTX54866 TRPM8 antibody.
Blue : Hoechst 33342 for nuclear staining
Dilution : 1:500 for Panel A ; 1:50 for Panel B

IHC-Fr analysis of rat DRG tissue using GTX54866 TRPM8 antibody. TRPM8 is expressed in DRG neurons. Hoechst 33342 is used as the counterstain.
Dilution : 1:100