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TRPV4 antibody

Anti-TRPV4 antibody used in Live cell imaging (LCI). GTX16630
Anti-TRPV4 antibody used in Western Blot (WB). GTX16630
Anti-TRPV4 antibody used in IHC (Frozen sections) (IHC-Fr). GTX16630
Anti-TRPV4 antibody used in Live cell imaging (LCI). GTX16630

Cat. No. GTX16630

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr, LCI

Reactivity

 Human, Rat
Package
50 μl ($639)
See all TRPV4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-Fr Assay dependent
LCI Assay dependent
Not tested in other applications.

Calculated MW

98 kDa. ( Note )

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Peptide (C)EDQSN(S)TVPSYPA(S)RD, corresponding to amino acid residues 647-662 (3rd extracellular loop) of rat TRPV4 (Accession  : Q9ERZ8).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transient receptor potential cation channel, subfamily V, member 4 , Otrpc4 , Vroac

Cellular Localization

Apical cell membrane

Background

a cation-selective channel; activated in response to systemic osmotic pressure [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Anti-TRPV4 antibody used in Live cell imaging (LCI). GTX16630

GTX16630 LCI Image

Live cell imaging analysis of live intact U-87 cells using GTX16630 TRPV4 antibody.
Red : Primary antibody
Blue : DAPI for nuclear staining
Dilution : 1:25

Anti-TRPV4 antibody used in Western Blot (WB). GTX16630

GTX16630 WB Image

WB analysis of rat brain lysate using GTX16630 TRPV4 antibody preincubated with or without immunogen peptide.
Dilution : 1:200

Anti-TRPV4 antibody used in IHC (Frozen sections) (IHC-Fr). GTX16630

GTX16630 IHC-Fr Image

IHC-Fr analysis of rat DRG tissue using GTX16630 TRPV4 antibody.
Panel A : TRPV4 labeling appears in neuronal cell bodies.
Panel B : Nuclear staining using DAPI.
Panel C : merged images of A and B.

Anti-TRPV4 antibody used in Live cell imaging (LCI). GTX16630

GTX16630 LCI Image

Live cell imaging analysis of live intact U-87 cells using GTX16630 TRPV4 antibody.
Panel A : Primary antibody
Panel B : Merge of panel A with the live view of the cell
Dilution : 1:25

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 639