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TRPV4 antibody

Anti-TRPV4 antibody used in Western Blot (WB). GTX54764
Anti-TRPV4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54764
Anti-TRPV4 antibody used in Western Blot (WB). GTX54764
Anti-TRPV4 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54764

Cat. No. GTX54764

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr, FACS, IP

Reactivity

 Human, Mouse, Rat
Package
50 μl ($639)
See all TRPV4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

98 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Peptide CDGHQQGYAPKWRAEDAPL, corresponding to amino acid residues 853-871 (Intracellular, C-terminus) of rat TRPV4 (Accession  : Q9ERZ8).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887884

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transient receptor potential cation channel, subfamily V, member 4 , Otrpc4 , Vroac

Cellular Localization

Apical cell membrane

Background

a cation-selective channel; activated in response to systemic osmotic pressure [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Anti-TRPV4 antibody used in Western Blot (WB). GTX54764

GTX54764 WB Image

WB analysis of rat brain lysate using GTX54764 TRPV4 antibody preincubated with or without immunogen peptide.
Dilution : 1:200

Anti-TRPV4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54764

GTX54764 ICC/IF Image

ICC/IF analysis of PFA-fixed rat DRG primary culture cells using GTX54764 TRPV4 antibody.
Panel A,D : Primary antibody
Panel B,E : DNA dye Hoechst 33342
Panel C,F : Merged images of panels A and B
Dilution : 1:500

Anti-TRPV4 antibody used in Western Blot (WB). GTX54764

GTX54764 WB Image

WB analysis of ND7/23 cell line lysate using GTX54764 TRPV4 antibody preincubated with or without immunogen peptide.
Dilution : 1:200

Anti-TRPV4 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54764

GTX54764 IHC-Fr Image

IHC-Fr analysis of rat DRG tissue using GTX54764 TRPV4 antibody.
Panel A : TRPV4 (red) in DRG neurons.
Panel B : Staining with mouse anti-Parvalbumin (green) in the same DRG section.
Panel C : Confocal merge of TRPV4 and Parvalbumin demonstrates colocalization.

REFERENCE

Application Reference

Zhao B et al. Front Physiol 2022; 13 : 927078 Activation of TRPV4 by lactate as a critical mediator of renal fibrosis in spontaneously hypertensive rats after moderate- and high-intensity exercise.
Gordon WC et al. Dermatol Ther (Heidelb) 2018; 8 (1) : 111-126 A Nonsteroidal Novel Formulation Targeting Inflammatory and Pruritus-Related Mediators Modulates Experimental Allergic Contact Dermatitis.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 639