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TRPV5 antibody

Anti-TRPV5 antibody used in Western Blot (WB). GTX54868
Anti-TRPV5 antibody used in IHC (Paraffin sections) (IHC-P). GTX54868
Anti-TRPV5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54868

Cat. No. GTX54868

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
50 μl ($639)
See all TRPV5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Predict Reactivity

Rabbit(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide (C)GLNLSEGDGEEVYHF, corresponding to amino acid residues 715-729 (Intracellular, C-terminus) of human TRPV5 (Accession Q9NQA5).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transient receptor potential cation channel subfamily V member 5 , CAT2 , ECAC1 , OTRPC3

Cellular Localization

Apical cell membrane

Background

This gene is a member of the transient receptor family and the TrpV subfamily. The calcium-selective channel encoded by this gene has 6 transmembrane-spanning domains, multiple potential phosphorylation sites, an N-linked glycosylation site, and 5 ANK repeats. This protein forms homotetramers or heterotetramers and is activated by a low internal calcium level. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-TRPV5 antibody used in Western Blot (WB). GTX54868

GTX54868 WB Image

WB analysis of rat kidney (lanes 1 and 3) and PC3 cell line (lanes 2 and 4) lysates using GTX54868 TRPV5 antibody preincubated with or without immunogen peptide.
Dilution : 1:200

Anti-TRPV5 antibody used in IHC (Paraffin sections) (IHC-P). GTX54868

GTX54868 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX54868 TRPV5 antibody. TRPV5 is expressed in collecting ducts (CD) in the renal cortex. Note that both proximal tubules (PT) and distal tubules (DT) are negative. Hematoxilin is used as the counterstain.
Dilution : 1:100

Anti-TRPV5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54868

GTX54868 ICC/IF Image

ICC/IF analysis of PFA-fixed PC-3 cells using GTX54868 TRPV5 antibody.
Panel A : Primary antibody
Panel B : Primary antibody prior incubated with immunogen peptide
Dilution : 1:20

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 639