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TRRAP antibody

Anti-TRRAP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129505
Anti-TRRAP antibody used in Western Blot (WB). GTX129505
Anti-TRRAP antibody used in ChIP assay (ChIP assay). GTX129505
Anti-TRRAP antibody used in Western Blot (WB). GTX129505
Anti-TRRAP antibody used in Immunoprecipitation (IP). GTX129505

Cat No. GTX129505

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IP, ChIP assay

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

438 kDa. ( Note )

Positive Control

Jurkat , Raji , NCI-H929 , 293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Rat, Bovine, Japanese Medaka(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human TRRAP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

transformation/transcription domain associated protein , PAF350/400 , PAF400 , STAF40 , TR-AP , Tra1

Cellular Localization

Nucleus

Background

Adapter protein, which is found in various multiprotein chromatin complexes with histone acetyltransferase activity (HAT), which gives a specific tag for epigenetic transcription activation. Component of the NuA4 histone acetyltransferase complex which is responsible for acetylation of nucleosomal histones H4 and H2A. Plays a central role in MYC transcription activation, and also participates in cell transformation by MYC. Required for p53/TP53-, E2F1- and E2F4-mediated transcription activation. Also involved in transcription activation mediated by the adenovirus E1A, a viral oncoprotein that deregulates transcription of key genes. Probably acts by linking transcription factors such as E1A, MYC or E2F1 to HAT complexes such as STAGA thereby allowing transcription activation. Probably not required in the steps following histone acetylation in processes of transcription activation. May be required for the mitotic checkpoint and normal cell cycle progression.

Database

Research Area

DATA IMAGES

Anti-TRRAP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129505

GTX129505 ICC/IF Image

TRRAP antibody detects TRRAP protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TRRAP protein stained by TRRAP antibody (GTX129505) diluted at 1:1000.
Blue: Hoechst 33342 staining.

Anti-TRRAP antibody used in Western Blot (WB). GTX129505

GTX129505 WB Image

TRRAP antibody detects TRRAP protein by western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
C. 30 μg NCI-H929 whole cell lysate/extract
5 % SDS-PAGE
TRRAP antibody (GTX129505) dilution: 1:500

Anti-TRRAP antibody used in ChIP assay (ChIP assay). GTX129505

GTX129505 ChIP assay Image

Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-TRRAP antibody. The precipitated DNA was detected by PCR with primer set targeting to nucleolin promoter.
ChIP experiment and primer designs are based on EMBO J. 2007 Jul 25;26(14):3332-45.

Anti-TRRAP antibody used in Western Blot (WB). GTX129505

GTX129505 WB Image

TRRAP antibody detects TRRAP protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
5 % SDS-PAGE
TRRAP antibody (GTX129505) dilution: 1:500

Anti-TRRAP antibody used in Immunoprecipitation (IP). GTX129505

GTX129505 IP Image

TRRAP antibody immunoprecipitates TRRAP protein in IP experiments.
IP samples: 293T whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of TRRAP protein by 4 μg TRRAP antibody (GTX129505)
C : Immunoprecipitation of TRRAP by 4 μg of TRRAP antibody (GTX129507)
5 % SDS-PAGE
The immunoprecipitated TRRAP protein was detected by TRRAP antibody (GTX129505) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169