APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:500 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Jurkat , Raji , NCI-H929 , 293T , A431 , HeLa , HepG2
Predict Reactivity
Mouse, Rat, Bovine, Japanese Medaka(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human TRRAP. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2886020
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
transformation/transcription domain associated protein , PAF350/400 , PAF400 , STAF40 , TR-AP , Tra1
Cellular Localization
Nucleus
Background
Adapter protein, which is found in various multiprotein chromatin complexes with histone acetyltransferase activity (HAT), which gives a specific tag for epigenetic transcription activation. Component of the NuA4 histone acetyltransferase complex which is responsible for acetylation of nucleosomal histones H4 and H2A. Plays a central role in MYC transcription activation, and also participates in cell transformation by MYC. Required for p53/TP53-, E2F1- and E2F4-mediated transcription activation. Also involved in transcription activation mediated by the adenovirus E1A, a viral oncoprotein that deregulates transcription of key genes. Probably acts by linking transcription factors such as E1A, MYC or E2F1 to HAT complexes such as STAGA thereby allowing transcription activation. Probably not required in the steps following histone acetylation in processes of transcription activation. May be required for the mitotic checkpoint and normal cell cycle progression.
Database
Research Area
DATA IMAGES
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GTX129505 WB Image
TRRAP antibody detects TRRAP protein by western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
C. 30 μg NCI-H929 whole cell lysate/extract
5 % SDS-PAGE
TRRAP antibody (GTX129505) dilution: 1:500
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GTX129505 WB Image
TRRAP antibody detects TRRAP protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
5 % SDS-PAGE
TRRAP antibody (GTX129505) dilution: 1:500
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GTX129505 ICC/IF Image
TRRAP antibody detects TRRAP protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TRRAP protein stained by TRRAP antibody (GTX129505) diluted at 1:1000.
Blue: Hoechst 33342 staining.
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GTX129505 ChIP assay Image
Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-TRRAP antibody. The precipitated DNA was detected by PCR with primer set targeting to nucleolin promoter.
ChIP experiment and primer designs are based on EMBO J. 2007 Jul 25;26(14):3332-45.
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GTX129505 IP Image
TRRAP antibody immunoprecipitates TRRAP protein in IP experiments.
IP samples: 293T whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of TRRAP protein by 4 μg TRRAP antibody (GTX129505)
C : Immunoprecipitation of TRRAP by 4 μg of TRRAP antibody (GTX129507)
5 % SDS-PAGE
The immunoprecipitated TRRAP protein was detected by TRRAP antibody (GTX129505) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
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REFERENCE
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