TRRAP antibody immunoprecipitates TRRAP protein in IP experiments.
IP samples: 293T whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of TRRAP protein by 4 μg TRRAP antibody (GTX129505)
C : Immunoprecipitation of TRRAP by 4 μg of TRRAP antibody (GTX129507)
5 % SDS-PAGE
The immunoprecipitated TRRAP protein was detected by TRRAP antibody (GTX129505) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-TRRAP antibody. The precipitated DNA was detected by PCR with primer set targeting to nucleolin promoter.

TRRAP antibody detects TRRAP protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TRRAP protein stained by TRRAP antibody (GTX129505) diluted at 1:1000.
Blue: Hoechst 33342 staining.

TRRAP antibody detects TRRAP protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
5 % SDS-PAGE
TRRAP antibody (GTX129505) dilution: 1:500

TRRAP antibody detects TRRAP protein by western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
C. 30 μg NCI-H929 whole cell lysate/extract
5 % SDS-PAGE
TRRAP antibody (GTX129505) dilution: 1:500