GeneTex
United States (US)

TSC2 antibody

GTX31286_2490_WB_20180221_18121410_694.jpg
GTX31286_2489_WB_20180221_18121410_892.jpg

Cat No. GTX31286

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ELISA
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

201 kDa. ( Note )

Positive Control

L1210 Cell Lysate(GTX27184) , Jurkat Cell Lysate(GTX27899) , Human Spleen Tissue Slide
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

TSC2 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human TSC2.The immunogen is located within amino acids 1450 - 1500 of TSC2.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tsc Complex Subunit 2,Lam,Ppp1R160,Tsc4,Tsc2

Background

Mutations in this gene lead to tuberous sclerosis complex. Its gene product is believed to be a tumor suppressor and is able to stimulate specific GTPases. The protein associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX31286_2490_WB_20180221_18121410_694.jpg

GTX31286 WB Image

WB analysis of L1210 cell lysate in the (A) absence and (B) presence of blocking peptide using GTX31286 TSC2 antibody.
Working concentration : 1 μg/ml

GTX31286_2489_WB_20180221_18121410_892.jpg

GTX31286 WB Image

WB analysis of Jurkat cell lysate using GTX31286 TSC2 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml