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TSC2 antibody

Anti-TSC2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX81899
Anti-TSC2 antibody used in Western Blot (WB). GTX81899
Anti-TSC2 antibody used in IHC (Paraffin sections) (IHC-P). GTX81899
Anti-TSC2 antibody used in Western Blot (WB). GTX81899
Anti-TSC2 antibody used in Western Blot (WB). GTX81899

Cat. No. GTX81899

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
400 μl ($399)
See all TSC2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:10-1:50
IHC-P 1:10-1:50
Not tested in other applications.

Calculated MW

201 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 1776-1805 amino acids from human Tuberin (TSC2).

Purification

Protein A purified, followed by peptide affinity purification.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

TSC complex subunit 2 , LAM , PPP1R160 , TSC4

Cellular Localization

Cytoplasm

Background

Mutations in this gene lead to tuberous sclerosis complex. Its gene product is believed to be a tumor suppressor and is able to stimulate specific GTPases. The protein associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-TSC2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX81899

GTX81899 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX81899 TSC2 antibody.
Green : Tuberin
Red : Actin filament
Blue : cell nucleus
Fixation : 4% paraformaldehyde (PFA) (20 min)
Permeabilization : Triton X-100 (0.1%, 10 min)
Dilution : 1:25

Anti-TSC2 antibody used in Western Blot (WB). GTX81899

GTX81899 WB Image

WB analysis of SH-SY5Y, MCF-7, PC-3, LNCaP, HeLa cell using GTX81899 TSC2 antibody.
Loading : 35ug per lane
Dilution : 1:1000

Anti-TSC2 antibody used in IHC (Paraffin sections) (IHC-P). GTX81899

GTX81899 IHC-P Image

IHC-P analysis of human hepatocarcinoma using GTX81899 TSC2 antibody.

Anti-TSC2 antibody used in Western Blot (WB). GTX81899

GTX81899 WB Image

WB analysis of PC-3, LNCaP, HeLa cell using GTX81899 TSC2 antibody.
Loading : 35ug per lane
Dilution : 1:1000

Anti-TSC2 antibody used in Western Blot (WB). GTX81899

GTX81899 WB Image

WB analysis of Ramos cell lysate (35ug/lane) using GTX81899 TSC2 antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399