GeneTex
United States (US)

TSFM antibody

GTX102238_40723_IHC_19011506_140.jpg
GTX102238_40723_WB_19011506_650.jpg
GTX102238_40723_20141205_IFA_19011506_984.jpg

Cat No. GTX102238

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

Jurkat , Raji , K562

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TSFM. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ts Translation Elongation Factor, Mitochondrial,Efts,Eftsmt,Tsfm

Cellular Localization

Mitochondrion

Background

Synthesis of the 13 mitochondrial-encoded proteins occurs on a dedicated mitochondrial translation apparatus similar to that found in prokaryotes and requires, in addition to the tRNAs and rRNAs encoded in mtDNA, the concerted action of several translation factors and a large number of mitochondrial ribosomal proteins, all of which are encoded by nuclear genes. The TSFM gene encodes a mitochondrial translation elongation factor (Smeitink et al., 2006 [PubMed 17033963]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX102238_40723_IHC_19011506_140.jpg

GTX102238 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using TSFM(GTX102238) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102238_40723_WB_19011506_650.jpg

GTX102238 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
B: K562
10% SDS PAGE
GTX102238 diluted at 1:1000

GTX102238_40723_20141205_IFA_19011506_984.jpg

GTX102238 ICC/IF Image

TSFM antibody detects TSFM protein at mitochondria by immunofluorescent analysis.
Sample: Hep G2 cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min.
Green: TSFM protein stained by TSFM antibody (GTX102238) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169