TSG101 ELISA Pair

Cat No. GTX500018

Application	ELISA, Sandwich ELISA
Reactivity	Human
Package	1 pair ($399)

See all TSG101 products

Datasheet

PRODUCT

Product Content

GTX118736

Type : Capture Antibody

Host : Rabbit

Conjugation : Unconjugated

Package : 50 μl

GTX70255

Type : Detection Antibody

Host : Mouse

Conjugation : Unconjugated

Package : 50 μl

APPLICATION

Application Note

We recommend using the capture antibody at a concentration of 5μg/mL and the detection antibody at a concentration of 1.5μg/mL. Optimal dilutions should be determined experimentally by the researcher.

PROPERTIES

Form

Liquid

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Tumor Susceptibility 101 , Tsg10 , Vps23 , Tsg101

Background

The protein encoded by this gene belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression. [provided by RefSeq]

Research Area

DATA IMAGES

GTX500018 ELISA Image

ELISA detection of TSG101 using GTX118736 for capture at a concentration of 5 μg/mL and GTX70255 for detection at a concentration of 1.5 μg/mL. The HRP-conjugated anti-mouse IgG antibody (GTX26789) was diluted at1:6,000-1:30,000 and used to detect the primary antibody.