APPLICATION
Application Note
We recommend using the capture antibody at a concentration of 5μg/mL and the detection antibody at a concentration of 1.5μg/mL. Optimal dilutions should be determined experimentally by the researcher.
PROPERTIES
Form
Liquid
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
Tumor Susceptibility 101 , Tsg10 , Vps23 , Tsg101
Background
The protein encoded by this gene belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression. [provided by RefSeq]
Research Area
DATA IMAGES
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GTX500018 ELISA Image
ELISA detection of TSG101 using GTX118736 for capture at a concentration of 5 μg/mL and GTX70255 for detection at a concentration of 1.5 μg/mL. The HRP-conjugated anti-mouse IgG antibody (GTX26789) was diluted at1:6,000-1:30,000 and used to detect the primary antibody.
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REFERENCE
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REVIEW
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