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TSG101 antibody [4A10]

Anti-TSG101 antibody [4A10] used in Western Blot (WB). GTX70255
Anti-TSG101 antibody [4A10] used in ELISA (ELISA). GTX70255
Anti-TSG101 antibody [4A10] used in Western Blot (WB). GTX70255
Anti-TSG101 antibody [4A10] used in Western Blot (WB). GTX70255
Anti-TSG101 antibody [4A10] used in Western Blot (WB). GTX70255
Anti-TSG101 antibody [4A10] used in Western Blot (WB). GTX70255
Anti-TSG101 antibody [4A10] used in Western Blot (WB). GTX70255
Anti-TSG101 antibody [4A10] used in IHC (Paraffin sections) (IHC-P). GTX70255
Anti-TSG101 antibody [4A10] used in IHC (Paraffin sections) (IHC-P). GTX70255
Anti-TSG101 antibody [4A10] used in IHC (Paraffin sections) (IHC-P). GTX70255
Anti-TSG101 antibody [4A10] used in Flow cytometry (FACS). GTX70255

Cat No. GTX70255

Host

Mouse

Clonality

Monoclonal

Clone Name

4A10

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, FACS, IP, ELISA, EM, IHC

Reactivity

Human, Mouse, Rat, Dog, Hamster, Monkey
Package
100 μl ($329)
PRODUCT

Summary

TSG101 antibody [4A10] is a well-validated and highly cited mouse monoclonal antibody that detects TSG101, a protein of 390 amino acids and a predicted molecular weight of 44 kDa that is a core constituent of the ESCRT-I complex. Reports have described it having both tumor suppressor and tumor enhancer activities. TSG101 is involved in exosome secretion and is commonly used as an exosome marker.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:500-1:1000
IHC-P 1:100-1:1000
FACS 1:25-1:200
IP Assay dependent
ELISA Assay dependent
EM 1:10
IHC 1:100
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

293T , K562 , THP-1 , HL-60 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , NIH-3T3 , JC , BCL-1

Product Note

Knockdown/Knockout validation was supported by references (PMID:27226592)

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Amino acids 167-374 of TSG101 protein expressed in E. coli.

Purification

Purified by antigen-affinity chromatography.
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_373239

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tumor susceptibility 101 , TSG10 , VPS23

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-TSG101 antibody [4A10] used in Western Blot (WB). GTX70255

GTX70255 WB Image

TSG101 antibody [4A10] detects TSG101 protein by western blot analysis.
A. 30 μg NIH-3T3 whole cell lysate/extract
B. 30 μg JC whole cell lysate/extract
C. 30 μg BCL-1 whole cell lysate/extract
10% SDS-PAGE
TSG101 antibody [4A10] (GTX70255) dilution: 1:500
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-TSG101 antibody [4A10] used in ELISA (ELISA). GTX70255

GTX70255 ELISA Image

ELISA detection of TSG101 using GTX118736 for capture at a concentration of 5 μg/mL and GTX70255 for detection at a concentration of 1.5 μg/mL.

Anti-TSG101 antibody [4A10] used in Western Blot (WB). GTX70255

GTX70255 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (GTX70255) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-TSG101 antibody [4A10] used in Western Blot (WB). GTX70255

GTX70255 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (GTX70255) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-TSG101 antibody [4A10] used in Western Blot (WB). GTX70255

GTX70255 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (GTX70255) diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-TSG101 antibody [4A10] used in Western Blot (WB). GTX70255

GTX70255 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (GTX70255) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-TSG101 antibody [4A10] used in Western Blot (WB). GTX70255

GTX70255 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (GTX70255) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-TSG101 antibody [4A10] used in IHC (Paraffin sections) (IHC-P). GTX70255

GTX70255 IHC-P Image

TSG101 antibody [4A10] detects TSG101 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
TSG101 stained by TSG101 antibody [4A10] (GTX70255) diluted at 1:50.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TSG101 antibody [4A10] used in IHC (Paraffin sections) (IHC-P). GTX70255

GTX70255 IHC-P Image

TSG101 antibody [4A10] detects TSG101 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
TSG101 stained by TSG101 antibody [4A10] (GTX70255) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TSG101 antibody [4A10] used in IHC (Paraffin sections) (IHC-P). GTX70255

GTX70255 IHC-P Image

TSG101 antibody [4A10] detects TSG101 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian cancer.
TSG101 stained by TSG101 antibody [4A10] (GTX70255) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TSG101 antibody [4A10] used in Flow cytometry (FACS). GTX70255

GTX70255 FACS Image

TSG101 antibody [4A10] (GTX70255) detects TSG101 protein by flow cytometry analysis.
Sample: THP-1 cell.
Black: Unlabelled sample was used as a control.
Red: TSG101 antibody [4A10] (GTX70255) dilution: 1:25.
Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.

REFERENCE

REVIEW

TSG101 antibody [4A10] Cat No. GTX70255

Rating ( Average 5 based on 11 users reviews)
Application
Western Blot(WB) ( Average 5 based on 10 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 23-Dec-2020
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Int J Mol Sci in 2020. PMID: 32230970
Date : Anonymous submitted on 23-Dec-2020
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2018. PMID: 29953492
Date : Anonymous submitted on 23-Dec-2020
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Cell Death Dis in 2018. PMID: 29743530
Date : Anonymous submitted on 23-Dec-2020
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Front Neurosci in 2019. PMID: 32009885
Date : Anonymous submitted on 23-Dec-2020
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Exp Clin Cancer Res in 2018. PMID: 30290833
Date : Anonymous submitted on 19-Nov-2020
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal BMC Biol in 2015. PMID: 25857663
Date : Anonymous submitted on 19-Nov-2020
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal BMC Biochem in 2007. PMID: 17880698
Date : Anonymous submitted on 19-Nov-2020
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal EMBO Mol Med in 2013. PMID: 23341130
Date : Anonymous submitted on 19-Nov-2020
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Virol J in 2008. PMID: 18267010
Date : Anonymous submitted on 19-Nov-2020
Score :
Application Tested : WB
Sample Species : Mk
Figure Note : The data was peer reviewed and published in the journal EMBO Mol Med in 2013. PMID: 23341130
Date : Anonymous submitted on 25-Nov-2019
Score :
Application Tested : WB
Sample Species : Hu, Rat
Sample : HEK293, NRK-52E cells
Amount used : 12.5 μg
Blocking : 0.25 % PVP-40 in TBST, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 18Hr
Application Reference
SDS
PBS.pdf