PRODUCT
Summary
TSG101 antibody [4A10] is a well-validated and highly cited mouse monoclonal antibody that detects TSG101, a protein of 390 amino acids and a predicted molecular weight of 44 kDa that is a core constituent of the ESCRT-I complex. Reports have described it having both tumor suppressor and tumor enhancer activities. TSG101 is involved in exosome secretion and is commonly used as an exosome marker.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:500-1:1000 |
1:100-1:1000 |
1:25-1:200 |
Assay dependent |
Assay dependent |
Assay dependent |
1:100 |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
293T , K562 , THP-1 , HL-60 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , NIH-3T3 , JC , BCL-1
Product Note
Knockdown/Knockout validation was supported by references (PMID:27226592)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.8 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Amino acids 167-374 of TSG101 protein expressed in E. coli.
Purification
Purified by antigen-affinity chromatography.
From tissue culture supernatant
Conjugation
Unconjugated
RRID
AB_373239
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
tumor susceptibility 101 , TSG10 , VPS23
Cellular Localization
Cytoplasm , Nucleus
Background
The protein encoded by this gene belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX70255 IHC-P Image
TSG101 antibody [4A10] detects TSG101 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.TSG101 stained by TSG101 antibody [4A10] (GTX70255) diluted at 1:50.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX70255 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (GTX70255) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX70255 IHC-P Image
TSG101 antibody [4A10] detects TSG101 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human ovarian cancer.TSG101 stained by TSG101 antibody [4A10] (GTX70255) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX70255 ELISA Image
ELISA detection of TSG101 using GTX118736 for capture at a concentration of 5 μg/mL and GTX70255 for detection at a concentration of 1.5 μg/mL.
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GTX70255 WB Image
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (GTX70255) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX70255 IHC-P Image
TSG101 antibody [4A10] detects TSG101 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.TSG101 stained by TSG101 antibody [4A10] (GTX70255) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX70255 FACS Image
TSG101 antibody [4A10] (GTX70255) detects TSG101 protein by flow cytometry analysis. Sample: THP-1 cell. Black: Unlabelled sample was used as a control. Red: TSG101 antibody [4A10] (GTX70255) dilution: 1:25. Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.
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GTX70255 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (GTX70255) diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX70255 WB Image
TSG101 antibody [4A10] detects TSG101 protein by western blot analysis. A. 30 μg NIH-3T3 whole cell lysate/extract B. 30 μg JC whole cell lysate/extract C. 30 μg BCL-1 whole cell lysate/extract 10% SDS-PAGE TSG101 antibody [4A10] (GTX70255) dilution: 1:500 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX70255 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (GTX70255) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
|
|
GTX70255 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (GTX70255) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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REFERENCE
REVIEW
TSG101 antibody [4A10]
Cat. No. GTX70255
Rating
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( Average 5 based on 27 users reviews)
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Application
Western Blot(WB)
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( Average 5 based on 24 users reviews)
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Immunocytochemistry/ Immunofluorescence(ICC/IF)
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( Average 5 based on 2 users reviews)
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IHC (Free Floating)(IHC (Free Floating))
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 24-Jul-2023
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the 2021 in Sci Rep.
PMID: 34127763
Date :
Anonymous submitted on 24-Jul-2023
Application Tested :
WB
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the 2020 in Eur J Neurosci.
PMID: 32939863
Date :
Anonymous submitted on 24-Jul-2023
Application Tested :
WB
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the 2020 in Eur J Neurosci.
PMID: 32939863
Date :
Anonymous submitted on 22-Feb-2022
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the Nat Commun in 2016.
PMID: 27882925
Date :
Anonymous submitted on 22-Feb-2022
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the Nat Commun in 2016.
PMID: 27882925
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal J Cell Sci in 2015.
PMID: 26002056
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal PLoS Biol in 2013.
PMID: 23874151
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal J Extracell Vesicles in 2012.
PMID: 24009879
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal Mol Neurodegener in 2017.
PMID: 28835279
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Acta Neuropathol Commun in 2017.
PMID: 28851452
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Acta Neuropathol Commun in 2017.
PMID: 28851452
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Sci Rep in 2017.
PMID: 28303002
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
IHC (Free Floating)
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal Mol Neurodegener in 2017.
PMID: 28835279
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
ICC/IF
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal Mol Neurodegener in 2017.
PMID: 28835279
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Acta Neuropathol Commun in 2017.
PMID: 28851452
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Nat Commun in 2015.
PMID: 26103923
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
ICC/IF
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal PLoS Pathog in 2015.
PMID: 25710462
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal PLoS One in 2017.
PMID: 28241021
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal Front Neurosci in 2019.
PMID: 32009885
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal PLoS One in 2018.
PMID: 29953492
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Cell Death Dis in 2018.
PMID: 29743530
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Sci Rep in 2017.
PMID: 28963552
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal PLoS Pathog in 2016.
PMID: 27203423
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Mk
Figure Note :
The data was peer reviewed and published in the journal Virol J in 2008.
PMID: 18267010
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Int J Mol Sci in 2020.
PMID: 32230970
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal J Exp Clin Cancer Res in 2018.
PMID: 30290833
Date :
Anonymous submitted on 25-Nov-2019
Application Tested :
WB
Sample Species :
Hu, Rat
Sample :
HEK293, NRK-52E cells
Amount used :
12.5 μg
Blocking :
0.25 % PVP-40 in TBST, 25°C, 1Hr
Primary Antibody :
1:2000, 4°C, 18Hr