GeneTex
United States (US)

TSG101 antibody [4A10]

KO/KD
GTX70255_41449_WB_M_18122101_336.jpg
GTX70255_TSG101_ELISA-PAIR_19012407_517.jpg
GTX70255_41449_20161110_WB_M_18122101_563.jpg
GTX70255_43180_20180420_WB_18122101_592.jpg
GTX70255_41449_20161201_WB_shRNA_watermark_18122101_903.jpg
GTX70255_43344_20180907_WB_19011519_723.jpg
GTX70255_43284_20181025_IHC-P_19011519_220.jpg
GTX70255_43354_20181207_IHC-P_19011519_359.jpg
GTX70255_43284_20181025_IHC-P_1_19011519_146.jpg

Cat No. GTX70255

Host Mouse
Clonality Monoclonal
Clone Name 4A10
Isotype IgG1
Application WB, ICC/IF, IHC-P, FACS, IP, ELISA, EM, IHC
Reactivity Human, Mouse, Rat, Hamster, Monkey
APPLICATION

Application Note

Suggested starting dilutions are as follows: WB: 1:500-1:3000. Suggested fixation for ICC/IF: 3-3.5% PFA with 0.05% saponin treatment. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Calculated MW

46 kDa. ( Note )

Positive Control

293T , K562 , THP-1 , HL-60 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , NIH-3T3 , JC , BCL-1

Specificity/Sensitivity

Knockdown/Knockout validation was supported by references (PMID:27226592)
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.36mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Amino acids 167-374 of TSG101 protein expressed in E. coli.

Purification

Purified by antigen-affinity chromatography.
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

TSG10, VPS23

Background

TSG101-4A10 recognizes TSG101 protein, the product of a recently identified Tumor Susceptibility Gene whose inactivation in mouse fibroblasts results in cell transformation and the ability of those cells to form tumors in nude mice. The TSG101 gene encodes a 46 kDa protein and contains a proline-rich domain and a luecine-zipper motif within a coiled-coil domain near the carboxyl terminus.

Database

Research Area

DATA IMAGES
GTX70255_41449_WB_M_18122101_336.jpg

GTX70255 WB Image

TSG101 antibody [4A10] detects TSG101 protein by western blot analysis.
A. 30 µg NIH-3T3 whole cell lysate/extract
B. 30 µg JC whole cell lysate/extract
C. 30 µg BCL-1 whole cell lysate/extract
10% SDS-PAGE
TSG101 antibody [4A10] (GTX70255) dilution: 1:500
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX70255_TSG101_ELISA-PAIR_19012407_517.jpg

GTX70255 ELISA Image

ELISA detection of TSG101 using GTX118736 for capture at a concentration of 5 µg/mL and GTX70255 for detection at a concentration of 1.5 µg/mL.

GTX70255_41449_20161110_WB_M_18122101_563.jpg

GTX70255 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (GTX70255) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX70255_43180_20180420_WB_18122101_592.jpg

GTX70255 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (GTX70255) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX70255_41449_20161201_WB_shRNA_watermark_18122101_903.jpg

GTX70255 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (GTX70255) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX70255_43344_20180907_WB_19011519_723.jpg

GTX70255 WB Image

K562 whole cell extracts (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (GTX70255) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX70255_43284_20181025_IHC-P_19011519_220.jpg

GTX70255 IHC-P Image

TSG101 antibody [4A10] detects TSG101 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
TSG101 stained by TSG101 antibody [4A10] (GTX70255) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX70255_43354_20181207_IHC-P_19011519_359.jpg

GTX70255 IHC-P Image

TSG101 antibody [4A10] detects TSG101 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
TSG101 stained by TSG101 antibody [4A10] (GTX70255) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX70255_43284_20181025_IHC-P_1_19011519_146.jpg

GTX70255 IHC-P Image

TSG101 antibody [4A10] detects TSG101 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian cancer.
TSG101 stained by TSG101 antibody [4A10] (GTX70255) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
Application Reference
SDS
PBS.pdf
Package List Price ($)
$ 319