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TSG101 antibody [4A10]

Anti-TSG101 antibody [4A10] used in Western Blot (WB). GTX70255
Anti-TSG101 antibody [4A10] used in ELISA (ELISA). GTX70255
Anti-TSG101 antibody [4A10] used in Western Blot (WB). GTX70255
Anti-TSG101 antibody [4A10] used in Western Blot (WB). GTX70255
Anti-TSG101 antibody [4A10] used in Western Blot (WB). GTX70255
Anti-TSG101 antibody [4A10] used in Flow cytometry (FACS). GTX70255
Anti-TSG101 antibody [4A10] used in Western Blot (WB). GTX70255
Anti-TSG101 antibody [4A10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70255
Anti-TSG101 antibody [4A10] used in Western Blot (WB). GTX70255
Anti-TSG101 antibody [4A10] used in IHC (Paraffin sections) (IHC-P). GTX70255
Anti-TSG101 antibody [4A10] used in IHC (Paraffin sections) (IHC-P). GTX70255
Anti-TSG101 antibody [4A10] used in IHC (Paraffin sections) (IHC-P). GTX70255
Anti-TSG101 antibody [4A10] used in Western Blot (WB). GTX70255

Cat No. GTX70255

Host Mouse
Clonality Monoclonal
Clone Name 4A10
Isotype IgG1
Application WB, ICC/IF, IHC-P, FACS, IP, ELISA, EM, IHC
Reactivity Human, Mouse, Rat, Hamster, Monkey
Package
100 μl ($319)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:500-1:1000
IHC-P 1:100-1:1000
FACS 1:25-1:200
IP Assay dependent
ELISA Assay dependent
EM 1:10
IHC 1:100
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

293T , K562 , THP-1 , HL-60 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , NIH-3T3 , JC , BCL-1

Specificity/Sensitivity

Knockdown/Knockout validation was supported by references (PMID:27226592)
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.74 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Amino acids 167-374 of TSG101 protein expressed in E. coli.

Purification

Purified by antigen-affinity chromatography.
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tumor Susceptibility 101 , Tsg10 , Vps23 , Tsg101

Background

TSG101-4A10 recognizes TSG101 protein, the product of a recently identified Tumor Susceptibility Gene whose inactivation in mouse fibroblasts results in cell transformation and the ability of those cells to form tumors in nude mice. The TSG101 gene encodes a 46 kDa protein and contains a proline-rich domain and a luecine-zipper motif within a coiled-coil domain near the carboxyl terminus.

Database

Research Area

DATA IMAGES
Anti-TSG101 antibody [4A10] used in Western Blot (WB). GTX70255

GTX70255 WB Image

TSG101 antibody [4A10] detects TSG101 protein by western blot analysis.
A. 30 µg NIH-3T3 whole cell lysate/extract
B. 30 µg JC whole cell lysate/extract
C. 30 µg BCL-1 whole cell lysate/extract
10% SDS-PAGE
TSG101 antibody [4A10] (GTX70255) dilution: 1:500
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-TSG101 antibody [4A10] used in ELISA (ELISA). GTX70255

GTX70255 ELISA Image

ELISA detection of TSG101 using GTX118736 for capture at a concentration of 5 µg/mL and GTX70255 for detection at a concentration of 1.5 µg/mL.

Anti-TSG101 antibody [4A10] used in Western Blot (WB). GTX70255

GTX70255 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (GTX70255) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-TSG101 antibody [4A10] used in Western Blot (WB). GTX70255

GTX70255 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (GTX70255) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-TSG101 antibody [4A10] used in Western Blot (WB). GTX70255

GTX70255 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (GTX70255) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-TSG101 antibody [4A10] used in Flow cytometry (FACS). GTX70255

GTX70255 FACS Image

TSG101 antibody [4A10] (GTX70255) detects TSG101 protein by flow cytometry analysis.
Sample: THP-1 cell.
Black: Unlabelled sample was used as a control.
Red: TSG101 antibody [4A10] (GTX70255) dilution: 1:25.
Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.

Anti-TSG101 antibody [4A10] used in Western Blot (WB). GTX70255

GTX70255 WB Image

Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (GTX70255) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-TSG101 antibody [4A10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70255

GTX70255 ICC/IF Image

Super-resolution images of Tsg101 at HIV-1 assembly sites.HeLa cells were transfected with HIV:HIVmCherry and endogenous Tsg101 was immunostained at 14 – 15 h post transfection. (A) Cells were imaged using TIRFM. A time projection of the TIRFM images are shown (HIVmCherry, left panel; Tsg101, middle panel; Overlay, magenta: HIVmCherry, green: Tsg101, right panel), scale bars: 10 μm. (B) Left panel. A zoomed image of the region highlighted in panel A. Scale bar: 2 μm. Middle panel. A zoomed image of the individual colocalizing Tsg101 cluster highlighted in the left panel. Scale bar: 500 nm. Right panel. A drift-corrected STORM image of the Tsg101 complex shown in the middle panel. Scale bar: 500 nm. (C) The size distribution of all Tsg101 structures colocalizing with HIVmCherry, revealing an average cluster size of 58 ± 7 nm. (D) Size distribution of all non-colocalizing Tsg101 clusters in cells expressing HIVmCherry revealing an average cluster size of 60 ± 15 nm or (E) or in cell expressing HIVmCherry late- revealing average cluster size of 58 ± 16 nm. Number N represents the number events contributing to the respective histogram.

Anti-TSG101 antibody [4A10] used in Western Blot (WB). GTX70255

GTX70255 WB Image

K562 whole cell extracts (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (GTX70255) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-TSG101 antibody [4A10] used in IHC (Paraffin sections) (IHC-P). GTX70255

GTX70255 IHC-P Image

TSG101 antibody [4A10] detects TSG101 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
TSG101 stained by TSG101 antibody [4A10] (GTX70255) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TSG101 antibody [4A10] used in IHC (Paraffin sections) (IHC-P). GTX70255

GTX70255 IHC-P Image

TSG101 antibody [4A10] detects TSG101 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
TSG101 stained by TSG101 antibody [4A10] (GTX70255) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TSG101 antibody [4A10] used in IHC (Paraffin sections) (IHC-P). GTX70255

GTX70255 IHC-P Image

TSG101 antibody [4A10] detects TSG101 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian cancer.
TSG101 stained by TSG101 antibody [4A10] (GTX70255) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TSG101 antibody [4A10] used in Western Blot (WB). GTX70255

GTX70255 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (GTX70255) diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE
REVIEW

TSG101 antibody [4A10] Cat No. GTX70255

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 25-Nov-2019
Score :
Application Tested : WB
Sample Species : Hu, Rat
Sample : HEK293, NRK-52E cells
Amount used : 12.5 μg
Blocking : 0.25 % PVP-40 in TBST, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 18Hr
Application Reference
SDS
PBS.pdf
Package List Price ($)
$ 319