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TSG101 antibody

Anti-TSG101 antibody used in IHC (Paraffin sections) (IHC-P). GTX64349
Anti-TSG101 antibody used in Western Blot (WB). GTX64349
Anti-TSG101 antibody used in Immunoprecipitation (IP). GTX64349

Cat. No. GTX64349

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all TSG101 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:100
IP 1:50 - 1:200
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human TSG101 (NP_006283.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tumor susceptibility 101 , TSG10 , VPS23

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-TSG101 antibody used in IHC (Paraffin sections) (IHC-P). GTX64349

GTX64349 IHC-P Image

IHC-P analysis of human liver damage tissue using GTX64349 TSG101 antibody.
Dilution : 1:100

Anti-TSG101 antibody used in Western Blot (WB). GTX64349

GTX64349 WB Image

WB analysis of various sample lysates using GTX64349 TSG101 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-TSG101 antibody used in Immunoprecipitation (IP). GTX64349

GTX64349 IP Image

IP analysis of HeLa cell lysate using GTX64349 TSG101 antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399