WB analysis of membrane-enriched extracts (30 μg lysate) of A-431 (Lane 1), IMR-32 (Lane 2) and Jurkat (Lane 3) using GTX25492 TSH receptor antibody [49].
Dilution : 1:250

ICC/IF analysis of A431 cells using GTX25492 TSH receptor antibody [49]. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature

ICC/IF analysis of A431 cells using GTX25492 TSH receptor antibody [49]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC