Unboiled TT whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TSH receptor antibody [HL3874] (GTX642112) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Unboiled various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TSH receptor antibody [HL3874] (GTX642112) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data are based on Human Protein Atlas program.