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TSLP antibody

Anti-TSLP antibody used in IHC (Paraffin sections) (IHC-P). GTX85059
Anti-TSLP antibody used in Western Blot (WB). GTX85059
Anti-TSLP antibody used in IHC (Paraffin sections) (IHC-P). GTX85059

Cat. No. GTX85059

Reference 8

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human, Mouse
Package
100 μg ($419)
See all TSLP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5 - 2 μg/mL
ICC/IF Assay dependent
IHC-P 2.5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

Positive Control

Human Brain Tissue Slide(GTX22203) , A20 Cell Lysate(GTX27180) , Human Brain Tissue Lysate(GTX27918)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

TSLP antibody was raised against a 19 amino acid synthetic peptide from near the center of human TSLP.The immunogen is located within amino acids 40 - 90 of TSLP.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_10726419

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

thymic stromal lymphopoietin

Cellular Localization

Secreted

Background

Thymic stromal lymphopoietin (TSLP) has recently been identified as an important factor capable of driving dendritic cell maturation and activation. TSLP is a four-helix-bundle cytokine that is expressed mainly by barrier epithelial cells and is a potent activator of several cell types such as myeloid dendritic cells. TSLP is involved in the positive selection of regulatory T cells, maintenance of peripheral CD4+ T cell homeostasis and the induction of CD4+ T cell-mediated allergic reaction. TSLP is also capable of supporting the growth of fetal liver and adult B cell progenitors and their differentiation to the IgM-positive stage of B cell development. Amino acid sequence analysis has shown poor homology between human and mouse TSLP although they exhibit similar biological functions and are expressed in similar tissues. Despite its predicted molecular weight, TSLP often migrates at a higher molecular weight in SDS-PAGE. At least two differentially spliced isoforms of TSLP are known to exist.

Database

Research Area

DATA IMAGES

Anti-TSLP antibody used in IHC (Paraffin sections) (IHC-P). GTX85059

GTX85059 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX85059 TSLP antibody.
Working concentration : 20 μg/ml

Anti-TSLP antibody used in Western Blot (WB). GTX85059

GTX85059 WB Image

WB analysis of A-20 cell lysate using GTX85059 TSLP antibody.
Working concentration : (A) 0.5, (B) 1, and (C) 2 μg/ml

Anti-TSLP antibody used in IHC (Paraffin sections) (IHC-P). GTX85059

GTX85059 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX85059 TSLP antibody.
Working concentration : 2.5 μg/ml

REFERENCE

Application Reference

Jang JH et al. Int J Mol Sci 2024; 25 (5) Chronic Gut Inflammation and Dysbiosis in IBS: Unraveling Their Contribution to Atopic Dermatitis Progression.
Garc?a LN et al. PLoS One 2020; 15 (5) : e0226233 Neonatal endotoxin stimulation is associated with a long-term bronchiolar epithelial?expression of innate immune and anti-allergic markers that attenuates the allergic response.
Application : IHC-P
Reactivity : Mouse
Qin W et al. Toxicol Appl Pharmacol 2020; 395 : 114981 Exposure to Diisononyl Phthalate Promotes Atopic March by Activating of NF-κB and p38 MAPK.
Application : IHC-P
Reactivity : Mouse
Kao JK et al. Kaohsiung J Med Sci 2020; (Epub) Subcutaneous injection of recombinant heat shock protein 70 ameliorates atopic dermatitis skin lesions in a mouse model.
Application : WB
Reactivity : Mouse
Wang E et al. Allergy 2019; (Epub) Benzo(a)pyrene Facilitates Dermatophagoides Group?1 (Der f 1)-Induced Epithelial Cytokine Release through Aryl Hydrocarbon Receptor in Asthma.
Application : WB, ICC/IF
Reactivity : Human
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REVIEW

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Application Reference
Jang JH et al. Int J Mol Sci 2024; 25 (5) Chronic Gut Inflammation and Dysbiosis in IBS: Unraveling Their Contribution to Atopic Dermatitis Progression.
Garc?a LN et al. PLoS One 2020; 15 (5):e0226233 Neonatal endotoxin stimulation is associated with a long-term bronchiolar epithelial?expression of innate immune and anti-allergic markers that attenuates the allergic response.
Application : IHC-P
Reactivity : Mouse
Qin W et al. Toxicol Appl Pharmacol 2020; 395:114981 Exposure to Diisononyl Phthalate Promotes Atopic March by Activating of NF-κB and p38 MAPK.
Application : IHC-P
Reactivity : Mouse
Kao JK et al. Kaohsiung J Med Sci 2020; (Epub) Subcutaneous injection of recombinant heat shock protein 70 ameliorates atopic dermatitis skin lesions in a mouse model.
Application : WB
Reactivity : Mouse
Wang E et al. Allergy 2019; (Epub) Benzo(a)pyrene Facilitates Dermatophagoides Group?1 (Der f 1)-Induced Epithelial Cytokine Release through Aryl Hydrocarbon Receptor in Asthma.
Application : WB, ICC/IF
Reactivity : Human
Kang J et al. Toxicology 2018; 404-405:49-58 Exposure to a combination of formaldehyde and DINP aggravated asthma-like pathology through oxidative stress and NF-κB activation.
Application : IHC-P
Reactivity : Mouse
Shen S et al. Food Chem Toxicol 2016; 99:60-69 Oral exposure to diisodecyl phthalate aggravates allergic dermatitis by oxidative stress and enhancement of thymic stromal lymphopoietin.
Application : IHC-P
Reactivity : Mouse
Wilson SR et al. Cell 2013; 155 (2):285-95 The epithelial cell-derived atopic dermatitis cytokine TSLP activates neurons to induce itch.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 419