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TSPAN3 antibody

Orthogonal
Anti-TSPAN3 antibody used in IHC (Paraffin sections) (IHC-P). GTX106868
Anti-TSPAN3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106868
Anti-TSPAN3 antibody used in Western Blot (WB). GTX106868
Anti-TSPAN3 antibody used in Western Blot (WB). GTX106868

Cat. No. GTX106868

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all TSPAN3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

293T , A431 , HeLa , Mouse liver

Predict Reactivity

Rat, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TSPAN3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11171584

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tetraspanin 3 , TM4-A , TM4SF8 , TSPAN-3

Cellular Localization

Membrane; Multi-pass membrane protein

Background

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The use of alternate polyadenylation sites has been found for this gene. Two alternative transcripts encoding different isoforms have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TSPAN3 antibody used in IHC (Paraffin sections) (IHC-P). GTX106868

GTX106868 IHC-P Image

TSPAN3 antibody detects TSPAN3 protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
TSPAN3 antibody (GTX106868) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TSPAN3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106868

GTX106868 ICC/IF Image

TSPAN3 antibody detects TSPAN3 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: TSPAN3 protein stained by TSPAN3 antibody (GTX106868) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-TSPAN3 antibody used in Western Blot (WB). GTX106868

GTX106868 WB Image

Sample (50 ug of whole cell lysate)
A: mouse kidney
12% SDS PAGE
GTX106868 diluted at 1:1000

Anti-TSPAN3 antibody used in Western Blot (WB). GTX106868

GTX106868 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TSPAN3 antibody (GTX106868) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169