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TSPYL1 antibody

Anti-TSPYL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX109809
Anti-TSPYL1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109809
Anti-TSPYL1 antibody used in Western Blot (WB). GTX109809

Cat. No. GTX109809

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all TSPYL1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

Molt-4 , Raji

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TSPYL1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952418

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

TSPY like 1 , TSPYL

Cellular Localization

Nucleus , nucleolus

Background

The protein encoded by this gene is found in the nucleolus and is similar to that of a family of genes on the Y-chromosome. This gene is intronless. Defects in this gene are a cause of sudden infant death with dysgenesis of the testes syndrome (SIDDT). [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TSPYL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX109809

GTX109809 IHC-P Image

TSPYL1 antibody detects TSPYL1 protein at nucleus in human breast cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human breast cancer.
TSPYL1 antibody (GTX109809) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TSPYL1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109809

GTX109809 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using TSPYL1(GTX109809) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.

Anti-TSPYL1 antibody used in Western Blot (WB). GTX109809

GTX109809 WB Image

Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
B: Raji
10% SDS PAGE
GTX109809 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169