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TST antibody

Orthogonal
Anti-TST antibody used in Western Blot (WB). GTX106257
Anti-TST antibody used in IHC (Paraffin sections) (IHC-P). GTX106257
Anti-TST antibody used in Western Blot (WB). GTX106257
Anti-TST antibody used in IHC (Paraffin sections) (IHC-P). GTX106257
Anti-TST antibody used in Western Blot (WB). GTX106257

Cat. No. GTX106257

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all TST products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.39 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TST. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2038185

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

thiosulfate sulfurtransferase , RDS

Cellular Localization

Mitochondrion matrix

Background

The product of this gene is a mitochondrial matrix enzyme that is encoded by the nucleus. It may play roles in cyanide detoxification, the formation of iron-sulfur proteins, and the modification of sulfur-containing enzymes. The gene product contains two highly conservative domains (rhodanese homology domains), suggesting these domains have a common evolutionary origin. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TST antibody used in Western Blot (WB). GTX106257

GTX106257 WB Image

Orthogonal

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TST antibody (GTX106257) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TST antibody used in IHC (Paraffin sections) (IHC-P). GTX106257

GTX106257 IHC-P Image

TST antibody detects TST protein at mitochondria on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
TST antibody (GTX106257) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TST antibody used in Western Blot (WB). GTX106257

GTX106257 WB Image

Mouse tissue extracts (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with TST antibody (GTX106257) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TST antibody used in IHC (Paraffin sections) (IHC-P). GTX106257

GTX106257 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using TST(GTX106257) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TST antibody used in Western Blot (WB). GTX106257

GTX106257 WB Image

Human tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with TST antibody (GTX106257) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169