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TST antibody

Orthogonal
Anti-TST antibody used in Western Blot (WB). GTX114858
Anti-TST antibody used in Western Blot (WB). GTX114858
Anti-TST antibody used in Western Blot (WB). GTX114858
Anti-TST antibody used in IHC (Paraffin sections) (IHC-P). GTX114858

Cat. No. GTX114858

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all TST products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.71 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TST. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620797

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

thiosulfate sulfurtransferase , RDS

Cellular Localization

Mitochondrion matrix

Background

The product of this gene is a mitochondrial matrix enzyme that is encoded by the nucleus. It may play roles in cyanide detoxification, the formation of iron-sulfur proteins, and the modification of sulfur-containing enzymes. The gene product contains two highly conservative domains (rhodanese homology domains), suggesting these domains have a common evolutionary origin. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TST antibody used in Western Blot (WB). GTX114858

GTX114858 WB Image

Human tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with TST antibody (GTX114858) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TST antibody used in Western Blot (WB). GTX114858

GTX114858 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TST antibody (GTX114858) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TST antibody used in Western Blot (WB). GTX114858

GTX114858 WB Image

Orthogonal

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TST antibody (GTX114858) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TST antibody used in IHC (Paraffin sections) (IHC-P). GTX114858

GTX114858 IHC-P Image

TST antibody detects TST protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
TST antibody (GTX114858) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Roderick N Carter et al. Cell Rep 2021; 37 (6) : 109958 The hepatic compensatory response to elevated systemic sulfide promotes diabetes.
Application : WB
Reactivity : Mouse
Marutani E et al. Nat Commun 2021; 12 (1) : 3108 Sulfide catabolism ameliorates hypoxic brain injury.
Application : WB
Reactivity : Mouse
Huang Y et al. J Virol 2020; 94 (23) Inositol-Requiring Enzyme 1α Promotes Zika Virus Infection through Regulation of Stearoyl Coenzyme A Desaturase 1-Mediated Lipid Metabolism.
Zivanovic J et al. Cell Metab 2019; 30 (6) : 1152-1170.e13 Selective Persulfide Detection Reveals Evolutionarily Conserved Antiaging Effects of S-Sulfhydration.
Shirozu K et al. Antioxid Redox Signal 2014; 20 (2) : 204-16 Cystathionine γ-lyase deficiency protects mice from galactosamine/lipopolysaccharide-induced acute liver failure.
Application : WB
Reactivity : Mouse
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169