GeneTex
  • Country / Location Selection

United States (US)

TTF2 antibody

Anti-TTF2 antibody used in Immunoprecipitation (IP). GTX109947
Anti-TTF2 antibody used in Western Blot (WB). GTX109947
Anti-TTF2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109947
Anti-TTF2 antibody used in IHC (Paraffin sections) (IHC-P). GTX109947

Cat. No. GTX109947

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

130 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.93 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TTF2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952424

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transcription termination factor 2 , HuF2 , ZGRF6

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes a member of the SWI2/SNF2 family of proteins, which play a critical role in altering protein-DNA interactions. The encoded protein has been shown to have dsDNA-dependent ATPase activity and RNA polymerase II termination activity. This protein interacts with cell division cycle 5-like, associates with human splicing complexes, and plays a role in pre-mRNA splicing. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TTF2 antibody used in Immunoprecipitation (IP). GTX109947

GTX109947 IP Image

TTF2 antibody immunoprecipitates TTF2 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of TTF2 protein by 2 μg of TTF2 antibody (GTX109947) 7.5% SDS-PAGE The immunoprecipitated TTF2 protein was detected by TTF2 antibody (GTX109947) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-TTF2 antibody used in Western Blot (WB). GTX109947

GTX109947 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with TTF2 antibody (GTX109947) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TTF2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109947

GTX109947 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TTF2(GTX109947) antibody at 1:500 dilution.

Anti-TTF2 antibody used in IHC (Paraffin sections) (IHC-P). GTX109947

GTX109947 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft , using TTF2(GTX109947) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

There are currently no references for TTF2 antibody (GTX109947). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for TTF2 antibody (GTX109947). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169