TTF2 antibody immunoprecipitates TTF2 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of TTF2 protein by 2 μg of TTF2 antibody (GTX109947) 7.5% SDS-PAGE The immunoprecipitated TTF2 protein was detected by TTF2 antibody (GTX109947) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Immunohistochemical analysis of paraffin-embedded A549 xenograft , using TTF2(GTX109947) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TTF2(GTX109947) antibody at 1:500 dilution.

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with TTF2 antibody (GTX109947) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.