TUFM antibody

Cat. No. GTX101763
Cat. No.  GTX101763
Anti-TUFM antibody used in Western Blot (WB). GTX101763
GTX101763 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TUFM antibody (GTX101763) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

1 / 11
Anti-TUFM antibody used in IHC (Paraffin sections) (IHC-P). GTX101763
GTX101763 IHC-P Image

TUFM antibody detects TUFM protein at mitochondria on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
TUFM antibody (GTX101763) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

2 / 11
Anti-TUFM antibody used in IHC (Paraffin sections) (IHC-P). GTX101763
GTX101763 IHC-P Image

TUFM antibody detects TUFM protein at mitochondria on H441 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded H441 xenograft.
TUFM antibody (GTX101763) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

3 / 11
Anti-TUFM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101763
GTX101763 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using TUFM(GTX101763) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.

4 / 11
Anti-TUFM antibody used in Western Blot (WB). GTX101763
GTX101763 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with TUFM antibody (GTX101763) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

5 / 11
Anti-TUFM antibody used in Western Blot (WB). GTX101763
GTX101763 WB Image

Sample (30 μg of whole cell lysate)
A:NIH-3T3 10% SDS PAGE
GTX101763 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

6 / 11
Anti-TUFM antibody used in Immunoprecipitation (IP). GTX101763
GTX101763 IP Image

Immunoprecipitation of TUFM protein from HepG2 whole cell extracts using 5 μg of TUFM antibody (GTX101763) or TUFM antibody (GTX101764).
Western blot analysis was performed using TUFM antibody (GTX101763).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

7 / 11
Anti-TUFM antibody used in Immunoprecipitation (IP). GTX101763
GTX101763 IP Image

TUFM antibody immunoprecipitates TUFM protein in IP experiments.
IP samples: HepG2 whole cell extract
A. 35 μg HepG2 whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of TUFM protein by 4 μg TUFM antibody (GTX101763)
10 % SDS-PAGE
The immunoprecipitated TUFM protein was detected by TUFM antibody (GTX101763) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

8 / 11
Anti-TUFM antibody used in Western Blot (WB). GTX101763
GTX101763 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TUFM antibody (GTX101763) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

9 / 11
Anti-TUFM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101763
GTX101763 ICC/IF Image

TUFM antibody detects TUFM protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TUFM stained by TUFM antibody (GTX101763) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

10 / 11
Anti-TUFM antibody used in Western Blot (WB). GTX101763
GTX101763 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TUFM antibody (GTX101763) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

11 / 11
Anti-TUFM antibody used in Western Blot (WB). GTX101763
Anti-TUFM antibody used in IHC (Paraffin sections) (IHC-P). GTX101763
Anti-TUFM antibody used in IHC (Paraffin sections) (IHC-P). GTX101763
Anti-TUFM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101763
Anti-TUFM antibody used in Western Blot (WB). GTX101763
Anti-TUFM antibody used in Western Blot (WB). GTX101763
Anti-TUFM antibody used in Immunoprecipitation (IP). GTX101763
Anti-TUFM antibody used in Immunoprecipitation (IP). GTX101763
Anti-TUFM antibody used in Western Blot (WB). GTX101763
Anti-TUFM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101763
Anti-TUFM antibody used in Western Blot (WB). GTX101763
  • Host
    Rabbit
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Applications
    WB ICC/IF IHC-P IP
  • Reactivity
    Human, Mouse
See all TUFM products