GeneTex
United States (US)

TUFM antibody

KO/KD
GTX101763_WB_19011506_866.jpg
GTX101763_39694_20141024_IHC_M_19011506_613.jpg
GTX101763_39694_IHC_19011506_331.jpg
GTX101763_WB_M_19011506_402.jpg
GTX101763_39694_IFA_19011506_388.jpg
GTX101763_39694_20141203_IP_19011506_772.jpg
GTX101763_39694_IP_19011506_844.jpg

Cat No. GTX101763

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

293T , H1299 , NIH-3T3

Specificity/Sensitivity

Knockdown/Knockout validation was supported by references (PMID:26990993)

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.42 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TUFM. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tu Translation Elongation Factor, Mitochondrial,Coxpd4,Ef-Tumt,Eftu,P43,Tufm

Cellular Localization

Mitochondrion

Background

This gene encodes a protein which participates in protein translation in mitochondria. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency resulting in lactic acidosis and fatal encephalopathy. A pseudogene has been identified on chromosome 17. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101763_WB_19011506_866.jpg

GTX101763 WB Image

Sample(30 µg of whole cell lysate)
A:293T
B:H1299
10% SDS PAGE
GTX101763 diluted at 1:1500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101763_39694_20141024_IHC_M_19011506_613.jpg

GTX101763 IHC-P Image

TUFM antibody detects TUFM protein at mitochondria on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
TUFM antibody (GTX101763) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101763_39694_IHC_19011506_331.jpg

GTX101763 IHC-P Image

TUFM antibody detects TUFM protein at mitochondria on H441 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded H441 xenograft.
TUFM antibody (GTX101763) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101763_WB_M_19011506_402.jpg

GTX101763 WB Image

Sample (30 µg of whole cell lysate)
A:NIH-3T3 10% SDS PAGE
GTX101763 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101763_39694_IFA_19011506_388.jpg

GTX101763 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using TUFM(GTX101763) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.

GTX101763_39694_20141203_IP_19011506_772.jpg

GTX101763 IP Image

Immunoprecipitation of TUFM protein from HepG2 whole cell extracts using 5 μg of TUFM antibody (GTX101763) or TUFM antibody (GTX101764).
Western blot analysis was performed using TUFM antibody (GTX101763).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX101763_39694_IP_19011506_844.jpg

GTX101763 IP Image

TUFM antibody immunoprecipitates TUFM protein in IP experiments.
IP samples: HepG2 whole cell extract
A. 35 μg HepG2 whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of TUFM protein by 4 μg TUFM antibody (GTX101763)
10 % SDS-PAGE
The immunoprecipitated TUFM protein was detected by TUFM antibody (GTX101763) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169