TUFM antibody

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TUFM antibody (GTX101763) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

TUFM antibody detects TUFM protein at mitochondria on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
TUFM antibody (GTX101763) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

TUFM antibody detects TUFM protein at mitochondria on H441 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded H441 xenograft.
TUFM antibody (GTX101763) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using TUFM(GTX101763) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with TUFM antibody (GTX101763) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (30 μg of whole cell lysate)
A:NIH-3T3 10% SDS PAGE
GTX101763 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunoprecipitation of TUFM protein from HepG2 whole cell extracts using 5 μg of TUFM antibody (GTX101763) or TUFM antibody (GTX101764).
Western blot analysis was performed using TUFM antibody (GTX101763).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

TUFM antibody immunoprecipitates TUFM protein in IP experiments.
IP samples: HepG2 whole cell extract
A. 35 μg HepG2 whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of TUFM protein by 4 μg TUFM antibody (GTX101763)
10 % SDS-PAGE
The immunoprecipitated TUFM protein was detected by TUFM antibody (GTX101763) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TUFM antibody (GTX101763) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

TUFM antibody detects TUFM protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TUFM stained by TUFM antibody (GTX101763) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TUFM antibody (GTX101763) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.