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TUFM antibody

KO/KD
Anti-TUFM antibody used in Western Blot (WB). GTX101763
Anti-TUFM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101763
Anti-TUFM antibody used in Western Blot (WB). GTX101763
Anti-TUFM antibody used in Western Blot (WB). GTX101763
Anti-TUFM antibody used in Immunoprecipitation (IP). GTX101763
Anti-TUFM antibody used in Immunoprecipitation (IP). GTX101763
Anti-TUFM antibody used in Western Blot (WB). GTX101763
Anti-TUFM antibody used in Western Blot (WB). GTX101763
Anti-TUFM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101763
Anti-TUFM antibody used in IHC (Paraffin sections) (IHC-P). GTX101763
Anti-TUFM antibody used in IHC (Paraffin sections) (IHC-P). GTX101763

Cat. No. GTX101763

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all TUFM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

NIH-3T3

Product Note

Knockdown/Knockout validation was supported by references (PMID:26990993)

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TUFM. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241420

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Tu translation elongation factor, mitochondrial , COXPD4 , EF-TuMT , EFTU , P43

Cellular Localization

Mitochondrion

Background

This gene encodes a protein which participates in protein translation in mitochondria. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency resulting in lactic acidosis and fatal encephalopathy. A pseudogene has been identified on chromosome 17. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TUFM antibody used in Western Blot (WB). GTX101763

GTX101763 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TUFM antibody (GTX101763) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TUFM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101763

GTX101763 ICC/IF Image

TUFM antibody detects TUFM protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TUFM stained by TUFM antibody (GTX101763) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-TUFM antibody used in Western Blot (WB). GTX101763

GTX101763 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TUFM antibody (GTX101763) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TUFM antibody used in Western Blot (WB). GTX101763

GTX101763 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TUFM antibody (GTX101763) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TUFM antibody used in Immunoprecipitation (IP). GTX101763

GTX101763 IP Image

TUFM antibody immunoprecipitates TUFM protein in IP experiments.
IP samples: HepG2 whole cell extract
A. 35 μg HepG2 whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of TUFM protein by 4 μg TUFM antibody (GTX101763)
10 % SDS-PAGE
The immunoprecipitated TUFM protein was detected by TUFM antibody (GTX101763) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-TUFM antibody used in Immunoprecipitation (IP). GTX101763

GTX101763 IP Image

Immunoprecipitation of TUFM protein from HepG2 whole cell extracts using 5 μg of TUFM antibody (GTX101763) or TUFM antibody (GTX101764).
Western blot analysis was performed using TUFM antibody (GTX101763).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-TUFM antibody used in Western Blot (WB). GTX101763

GTX101763 WB Image

Sample (30 μg of whole cell lysate)
A:NIH-3T3 10% SDS PAGE
GTX101763 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TUFM antibody used in Western Blot (WB). GTX101763

GTX101763 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with TUFM antibody (GTX101763) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TUFM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101763

GTX101763 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using TUFM(GTX101763) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.

Anti-TUFM antibody used in IHC (Paraffin sections) (IHC-P). GTX101763

GTX101763 IHC-P Image

TUFM antibody detects TUFM protein at mitochondria on H441 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded H441 xenograft.
TUFM antibody (GTX101763) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TUFM antibody used in IHC (Paraffin sections) (IHC-P). GTX101763

GTX101763 IHC-P Image

TUFM antibody detects TUFM protein at mitochondria on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
TUFM antibody (GTX101763) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Avolio R et al. Genome Res 2023; 33 (8) : 1242-1257 Cytosolic and mitochondrial translation elongation are coordinated through the molecular chaperone TRAP1 for the synthesis and import of mitochondrial proteins.
Avolio R et al. bioRxiv 2023; Cytosolic and mitochondrial translation elongation are coordinated through the molecular chaperone TRAP1 for the synthesis and import of mitochondrial proteins.
Application : WB
Reactivity : Human
Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : WB, ICC/IF, IP
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169