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TUFM antibody

Anti-TUFM antibody used in Immunoprecipitation (IP). GTX55825
Anti-TUFM antibody used in IHC (Paraffin sections) (IHC-P). GTX55825
Anti-TUFM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55825
Anti-TUFM antibody used in Western Blot (WB). GTX55825

Cat. No. GTX55825

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all TUFM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:10 - 1:100
IHC-P 1:50 - 1:200
IP 1:50 - 1:200
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 186-455 of human TUFM (NP_003312.3).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Tu translation elongation factor, mitochondrial , COXPD4 , EF-TuMT , EFTU , P43

Cellular Localization

Mitochondrion

Background

This gene encodes a protein which participates in protein translation in mitochondria. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency resulting in lactic acidosis and fatal encephalopathy. A pseudogene has been identified on chromosome 17. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-TUFM antibody used in Immunoprecipitation (IP). GTX55825

GTX55825 IP Image

IP analysis of 293T cell lysate using GTX55825 TUFM antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000

Anti-TUFM antibody used in IHC (Paraffin sections) (IHC-P). GTX55825

GTX55825 IHC-P Image

IHC-P analysis of human esophageal cancer tissue using GTX55825 TUFM antibody.
Dilution : 1:100

Anti-TUFM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55825

GTX55825 ICC/IF Image

ICC/IF analysis of MCF7 cells using GTX55825 TUFM antibody.
Blue : DAPI

Anti-TUFM antibody used in Western Blot (WB). GTX55825

GTX55825 WB Image

WB analysis of various sample lysates using GTX55825 TUFM antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399